製品の概要

  • 製品名Anti-Jarid2 antibody
    Jarid2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Jarid2
  • 特異性This antibody is specific for Jarid2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic peptide made to an N-terminal portion of the human Jarid2 protein, between residues 1-100

  • ポジティブ・コントロール
    • E16 mouse brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2 µg/ml. Detects a band of approximately 139 kDa (predicted molecular weight: 139 kDa).

ターゲット情報

  • 機能Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.
  • 組織特異性During embryogenesis, predominantly expressed in neurons and particularly in dorsal root ganglion cells.
  • 配列類似性Contains 1 ARID domain.
    Contains 1 JmjC domain.
    Contains 1 JmjN domain.
  • ドメインThe ARID domain is required to target the PRC2 complex to its target genes.
    The GSGFP motif is required for the interaction with SUZ12.
  • 細胞内局在Nucleus. Colocalizes with the PRC2 complex on chromatin.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JARD2 antibody
    • JARD2_HUMAN antibody
    • JARID2 antibody
    • JMJ antibody
    • Jumonji AT rich interactive domain 2 antibody
    • Jumonji homolog antibody
    • Jumonji like protein antibody
    • Jumonji protein antibody
    • Jumonji/ARID domain containing protein 2 antibody
    • Jumonji/ARID domain-containing protein 2 antibody
    • Protein Jumonji antibody
    see all

Anti-Jarid2 antibody 画像

  • Anti-Jarid2 antibody (ab48137) at 2 µg/ml + E16 mouse brain lysate at 50 µg

    Predicted band size : 139 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,80 kDa. We are unsure as to the identity of these extra bands.

Anti-Jarid2 antibody (ab48137) 使用論文

This product has been referenced in:
  • van Heeringen SJ  et al. Principles of nucleation of H3K27 methylation during embryonic development. Genome Res 24:401-10 (2014). ChIP ; Xenopus tropicalis . Read more (PubMed: 24336765) »
  • Sourvinos G  et al. The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection. PLoS Pathog 10:e1004136 (2014). WB ; Human . Read more (PubMed: 24830456) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 800000 cells
Specification HeLa
Gel Running Conditions Reduced Denaturing (6%)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 Nov 08 2011

I am sorry to hear that you have been experiencing problems with ab48137 in WB. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the a...

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