The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20581148
ab18392 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 19635909
1/50 - 1/100. Use under non reducing condition. Predicted molecular weight: 46 kDa.
1/5 - 1/20.
Use at an assay dependent concentration.
The Japanese encephalitis viral genome encodes 7 non-structural proteins NS1-NS5. NS1 contains N-linked carbohydrate chains at positions 130 and 207. It is not incorporated into the virion but exists in the host cell, on the cell surface and can also be extracellular.
This antibody recognsies 2 forms of NS1 - NS1 and NS1' (46 and 53 kDa respectively). NS1' is thought to be formed when NS1 is cleaved from NS2A at an alternative site. Both NS1 and NS1' exist as dimers in untreated samples but are dissociated into monomers when samples are boiled.
Li XF et al. Development of a chimeric Zika vaccine using a licensed live-attenuated flavivirus vaccine as backbone. Nat Commun9:673 (2018).
Read more (PubMed: 29445153) »
Sharma N et al. Japanese Encephalitis Virus exploits the microRNA-432 to regulate the expression of Suppressor of Cytokine Signaling (SOCS) 5. Sci Rep6:27685 (2016).
Japanese encephalitis virus
Read more (PubMed: 27282499) »