Janus Green cell normalization stain (ab111622)

製品の概要

  • 製品名Janus Green cell normalization stain
  • アプリケーション適用あり: In-Cell ELISAmore details
  • 特記事項Cells fixed to a solid surface (typically in microplate wells) can be stained by adding 1X Janus Green Stain. Incubate cells for 5 minutes at room temperature. Dye should be removed and washed 5 times in ultrapure water or until excess dye is removed. The last wash should be removed and add 0.1 mL 0.5 M HCl per well and incubate for 10 minutes. Shake the plate for 10 seconds, and measure the OD 595 nm using a standard microplate spectrophotometer.

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at +4°C.
  • バッファーConstituents: 0.02% Monobasic dihydrogen potassium phosphate, 0.216% Dibasic monohydrogen sodium phosphate, 0.02% Potassium chloride, 0.81% Sodium chloride, Water, 0.3% Janus Green B
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  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab111622 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    In-Cell ELISA Use at an assay dependent dilution. 50 µL/ well fixed cells

    Janus Green cell normalization stain (ab111622) 使用論文

    ab111622 has not yet been referenced specifically in any publications.

    Product Wall

    Yes, the OD 595 can be read directly on the plate where the cells were grown and the assay was performed. The HCL will disrupt the cells and the janus green (which stains the nuclei) will be released into the well. No need to spin or move the HCL to a ...

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    Thank you for your enquiry,

    We recommend to measure the assays results at OD595 nm using a standard microplate spectrophotometer. We will be amending the datasheet to make this clearer.

    Whole Cell Staining with Janus Green:
    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"