製品の概要

  • 製品名Anti-JAK3 antibody [EP909Y]
    JAK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP909Y] to JAK3
  • アプリケーション適用あり: WB, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human JAK3.

  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 125 kDa.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.

ターゲット情報

  • 機能Tyrosine kinase of the non-receptor type, involved in the interleukin-2 and interleukin-4 signaling pathway. Phosphorylates STAT6, IRS1, IRS2 and PI3K.
  • 組織特異性In NK cells and an NK-like cell line but not in resting T-cells or in other tissues. The S-form is more commonly seen in hematopoietic lines, whereas the B-form is detected in cells both of hematopoietic and epithelial origins.
  • 関連疾患Defects in JAK3 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-negative (T(-)B(+)NK(-) SCID) [MIM:600802]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • ドメインPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
  • 翻訳後修飾Tyrosine phosphorylated in response to IL-2 and IL-4.
  • 細胞内局在Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 2.7.10.2 antibody
    • JAK 3 antibody
    • JAK L antibody
    • JAK-3 antibody
    • Jak3 antibody
    • JAK3 HUMAN antibody
    • JAK3_HUMAN antibody
    • JAKL antibody
    • Janus kinase 3 (a protein tyrosine kinase, leukocyte) antibody
    • Janus kinase 3 antibody
    • Janus Kinase3 antibody
    • L JAK antibody
    • L-JAK antibody
    • Leukocyte janus kinase antibody
    • LJAK antibody
    • Protein tyrosine kinase leukocyte antibody
    • Tyrosine protein kinase JAK3 antibody
    • Tyrosine-protein kinase JAK3 antibody
    see all

Anti-JAK3 antibody [EP909Y] 画像

  • Anti-JAK3 antibody [EP909Y] (ab45141) at 1/2000 dilution + TF-1 cell lysate

    Predicted band size : 125 kDa
    Observed band size : 125 kDa
  • Overlay histogram showing Jurkat cells stained with ab45141 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45141, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-JAK3 antibody [EP909Y] (ab45141) 使用論文

ab45141 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab45141.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"