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Synthetic peptide (Human) derived from JAK2 around the phosphorylation site of Tyrosine 221.
Our Abpromise guarantee covers the use of ab39636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21420414|
|WB||1/500 - 1/1000.|
Formalin-fixed, paraffin-embedded dog lung tissue stained fo JAK2 using ab39636 at 1/400 dilution in immunohistochemical analysis. Primary antibosy was incubated for 30 minutes at 20°C.
Heat mediated antigen retrieval was performed using 1 mM EDTA buffer pH 8.