製品の概要

  • 製品名Anti-JAK1 antibody
    JAK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to JAK1
  • 特異性Detects endogenous levels of JAK1 protein
  • アプリケーション適用あり: ICC/IF, IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic non - phosphopeptide (Human) from around the phosphorylation site of Tyrosine 1022

  • ポジティブ・コントロール
    • Human breast carcinoma tissue. Extracts from MCF7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab47435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).

ターゲット情報

  • 機能Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
  • 組織特異性Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
  • ドメインPossesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.
    The FERM domain mediates interaction with JAKMIP1.
  • 細胞内局在Endomembrane system. Wholly intracellular, possibly membrane associated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • JAK 1 antibody
    • JAK 1A antibody
    • JAK 1B antibody
    • JAK-1 antibody
    • JAK1 antibody
    • JAK1_HUMAN antibody
    • JAK1A antibody
    • JAK1B antibody
    • Janus kinase 1 (a protein tyrosine kinase) antibody
    • Janus kinase 1 antibody
    • JTK3 antibody
    • Tyrosine protein kinase JAK 1 antibody
    • Tyrosine protein kinase JAK1 antibody
    • Tyrosine-protein kinase JAK1 antibody
    see all

Anti-JAK1 antibody 画像

  • Immunohistochemical staining of paraffin embedded human breast carcinoma tissue using ab47435 at dilution of 1/50-1/100. Right panel treated with peptide and left panel untreated
  • All lanes : Anti-JAK1 antibody (ab47435) at 1/500 dilution

    Lane 1 : MCF7 cell extract treated with EGF
    Lane 2 : MCF7 cell extract treated with EGF with peptide


    Predicted band size : 132 kDa
  • ICC/IF image of ab47435 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47435, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-JAK1 antibody (ab47435) 使用論文

This product has been referenced in:
  • Murphy MJ  et al. Leukemia Inhibitory Factor is Necessary for Ovulation in Female Rhesus Macaques. Endocrinology N/A:en20161283 (2016). IHC ; Macaque Monkey . Read more (PubMed: 27571132) »
  • El Jamal SM  et al. Interferon gamma-induced apoptosis of head and neck squamous cell carcinoma is connected to indoleamine-2,3-dioxygenase via mitochondrial and ER stress-associated pathways. Cell Div 11:11 (2016). WB ; Human . Read more (PubMed: 27486476) »

See all 3 Publications for this product

Product Wall

Please accept my apologies for you having trouble with ab47435. The anti-JAK1 antibody is covered under our Abpromise, which states that we guarantee all of our antibodies for 6 months to work as stated on the datasheet. In the case of ab47435, it is g...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"