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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Jagged1 aa 1150-1250 (C terminal).
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab109536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 134 kDa.|
|IHC-P||1/200 - 1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
|IHC-Fr||Use at an assay dependent concentration. PubMed: 24901044|
Immunohistochemical staining of paraffin embedded human kidney using purified ab109536 at a dilution of 1/300. A pre-diluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin.
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemical staining of Jagged1 in paraffin-embedded bladder cancer tissue using unpurified ab109536 at a dilution of 1/250.