製品の概要

  • 製品名Anti-Jagged 2 antibody
    Jagged 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Jagged 2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Synthetic peptide derived from a region within N terminal amino acids 1-50 (MRAQGRGRLP RRLLLLLALW VQAARPMGYF ELQLSALRNV NGELLSGACC) of Human Jagged 2

  • ポジティブ・コントロール
    • Jurkat membrane lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 133 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性Jagged 2 is a Notch ligand involved in the mediation of Notch signaling. Jagged 2 is expressed in heart, placenta and skeletal muscle and to a lesser extend in pancreas. Very low expression in brain, lung, liver and kidney. The Notch signaling pathway is an intercellular signaling mechanism that is essential for proper embryonic development. Members of the Notch gene family encode transmembrane receptors that are critical for various cell fate decisions. Jagged 2 is one of several ligands that activate Notch and related receptors. Two transcript variants encoding different isoforms have been found for Jagged 2.
  • 細胞内局在Cell Membrane
  • 参照データベース
  • 別名
    • D12Ggc2e antibody
    • HJ 2 antibody
    • HJ2 antibody
    • JAG 2 antibody
    • JAG2 antibody
    • Jagged 2 precursor antibody
    • Jagged2 antibody
    • Protein jagged 2 antibody
    • SER2 antibody
    • Syndactylism antibody
    see all

Anti-Jagged 2 antibody 画像

  • Anti-Jagged 2 antibody (ab80671) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
    Additional bands at : <40 kDa. We are unsure as to the identity of these extra bands.

Anti-Jagged 2 antibody (ab80671) 使用論文

ab80671 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Embryos -GD9)
Gel Running Conditions Reduced Denaturing (10)
Loading amount 20 µg
Treatment hydroxyurea
Specification Embryos -GD9
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Nazem El Husseini

Verified customer

投稿 May 16 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"