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Western blot for Jagged 2 antibody (ab10556) at 1/500 tested on the following tissue lysates:
Lane 1: Mouse Brain
Lane 2: Mouse Lung
Lane 3: Rat Brain
Lane 4: Rat Lung
Lane 5: Mouse Brain Day 0
Secondary ab: Goat anti-rabbit IgG ab6721 (1/5000)
Exposure time: 10 minutes
Expected molecular weight: 130 kDa
Cell lysates at 20
NB: This image shows that is was not possible to detect Jagged 2 by WB in the lysates tested. This maybe due to low expression/abundance of Jagged 2 compounded by the fact that Jagged 2 expression is developmentally regulated and induced making it difficult to detect in whole tissue homogenates. In favour of the Jagged 2 antibody (ab10556), this WB shows relatively low background and minimal cross reactivity in tissue lysate.
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