Anti-ITM2B 抗体 (ab101522)
Key features and details
- Rabbit polyclonal to ITM2B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ITM2B antibody
ITM2B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ITM2B -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Pig -
免疫原
Recombinant fragment corresponding to Human ITM2B aa 65-234.
Database link: Q9Y287 -
ポジティブ・コントロール
- WB: HepG2, HCT116 whole cell lysate. Mouse brain lysate. Rat brain tissue extract. ICC/IF: HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101522の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 30 kDa.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 30 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Functions as a protease inhibitor. Plays a role in APP processing regulating the physiological production of the beta amyloid peptide. Restricts docking of gamma-secretase to APP and access of alpha- and beta-secretase to their cleavage APP sequence. -
組織特異性
Expressed in brain and in other tissues. -
関連疾患
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 1 (CAA-ITM2B1) [MIM:176500]. A disorder characterized by amyloid deposition in the walls of cerebral blood vessels and neurodegeneration in the central nervous system. Cerebral amyloid angiopathy, non-neuritic and perivascular plaques and neurofibrillary tangles are the predominant pathological lesions. Clinical features include progressive mental deterioration, spasticity and muscular rigidity.
Defects in ITM2B are a cause of cerebral amyloid angiopathy ITM2B-related type 2 (CAA-ITM2B2) [MIM:117300]; also known as heredopathia ophthalmo-oto-encephalica. A disorder characterized by amyloid deposition in the walls of the blood vessels of the cerebrum, choroid plexus, cerebellum, spinal cord and retina. Plaques and neurofibrillary tangles are observed in the hippocampus. Clinical features include progressive ataxia, dementia, cataracts and deafness. -
配列類似性
Belongs to the ITM2 family.
Contains 1 BRICHOS domain. -
翻訳後修飾
The C-terminal part of the ectodomain is processed by furin and related proteases producing a secreted peptide of 4 to 5 kDa. For the ABRI and ADAN variants the C-terminal secreted peptide is larger and may produce amyloid fibrils responsible for neuronal dysfunction and dementia. The remaining part of the ectodomain containing the BRICHOS domain is cleaved by ADAM10 and is secreted as a peptide of 25 kDa. The membrane-bound N-terminal fragment (NTF) of 22 kDa is further proteolytically processed by SPPL2A and SPPL2B through regulated intramembrane proteolysis producing a secreted peptide (BRI2C) and an intracellular domain (ICD) released in the cytosol. -
細胞内局在
Golgi apparatus membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 510575 Cow
- Entrez Gene: 9445 Human
- Entrez Gene: 16432 Mouse
- Entrez Gene: 595120 Pig
- Entrez Gene: 290364 Rat
- Omim: 603904 Human
- SwissProt: Q3T0P7 Cow
- SwissProt: Q9Y287 Human
see all -
別名
- ABRI antibody
- ABri/ADan amyloid peptide antibody
- BRI 2 antibody
see all
画像
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All lanes : Anti-ITM2B antibody (ab101522) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 30 kDa -
HeLa cells stained for ITM2B (green) using ab101522 at 1/200 dilution in ICC/IF.
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Anti-ITM2B antibody (ab101522) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 30 kDa12% SDS-PAGE
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Anti-ITM2B antibody (ab101522) at 1/1000 dilution + Mouse brain lysate at 50 µg
Predicted band size: 30 kDa12 % SDS-PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab101522 は論文での使用が確認できていません。