製品の概要

  • 製品名Anti-ITK antibody [Y401]
    ITK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y401] to ITK
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues in the N terminus of human Emtk/ITK/Tsk.

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate. IHC-P: Human lymph node.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • Concentration information loading...
  • ポリ/モノモノクローナル
  • クローン名Y401
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 68 kDa (predicted molecular weight: 72 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Plays a role in T-cell proliferation and differentiation.
    • 組織特異性T-cell lines and natural killer cell lines.
    • 関連疾患Defects in ITK are the cause of lymphoproliferative syndrome EBV-associated autosomal type 1 (LPSA1) [MIM:613011]. LPSA1 is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Inadequate immune response to EBV can have a fatal outcome. Clinical features include splenomegaly, lymphadenopathy, anemia, thrombocytopenia, pancytopenia, recurrent infections. There is an increased risk for lymphoma.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
      Contains 1 Btk-type zinc finger.
      Contains 1 PH domain.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 細胞内局在Cell membrane. Localizes to cell surface receptors in the plasma membrane after stimulation with respective receptors (TCR, CD28, CD2) in T-cells.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EMT antibody
      • Homolog of mouse T cell itk/tsk antibody
      • IL 2 inducible T cell kinase antibody
      • IL2 inducible T cell kinase antibody
      • Interleukin 2 inducible T cell kinase antibody
      • Itk antibody
      • ITK_HUMAN antibody
      • Kinase EMT antibody
      • LPFS1 antibody
      • LYK antibody
      • MGC126257 antibody
      • MGC126258 antibody
      • PSCTK 2 antibody
      • PSCTK2 antibody
      • T cell specific kinase antibody
      • T-cell-specific kinase antibody
      • TSK antibody
      • Tyrosine protein kinase antibody
      • Tyrosine protein kinase ITK/TSK antibody
      • Tyrosine protein kinase Lyk antibody
      • Tyrosine-protein kinase ITK/TSK antibody
      • Tyrosine-protein kinase Lyk antibody
      see all

    Anti-ITK antibody [Y401] 画像

    • Anti-ITK antibody [Y401] (ab32039) at 1/5000 dilution + Jurkat cell lysate

      Predicted band size : 72 kDa
      Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    • Ab32039 at a 1/250 dilution, staining ITK in paraffin embedded human lymph node tissue by Immunohistochemistry.
    • Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32039, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (1µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    プロトコール

    Anti-ITK antibody [Y401] (ab32039) 使用論文

    This product has been referenced in:
    • Carson CC  et al. IL2 Inducible T-cell Kinase, a Novel Therapeutic Target in Melanoma. Clin Cancer Res 21:2167-76 (2015). Human . Read more (PubMed: 25934889) »
    • Stepensky P  et al. IL-2-inducible T-cell kinase deficiency: clinical presentation and therapeutic approach. Haematologica 96:472-6 (2011). WB ; Human . Read more (PubMed: 21109689) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"