The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 129 kDa. for the full length protein.
Use at an assay dependent concentration.
1/100. (see Abreview)
Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells.
Isoforms containing segment A are predominantly expressed in skeletal muscle. Isoforms containing segment B are abundantly expressed in skeletal muscle, moderately in cardiac muscle, small intestine, colon, ovary and prostate and weakly in lung and testes. Isoforms containing segment X2D are expressed at low levels in fetal and adult skeletal muscle and in cardiac muscle, but are not detected in myoblasts and myotubes. In muscle fibers isoforms containing segment A and B are expressed at myotendinous and neuromuscular junctions; isoforms containing segment C are expressed at neuromuscular junctions and at extrasynaptic sites. Isoforms containing segments X1 or X2 or, at low levels, X1X2 are expressed in fetal and adult skeletal muscle (myoblasts and myotubes) and cardiac muscle.
Defects in ITGA7 are the cause of muscular dystrophy congenital due to integrin alpha-7 deficiency (MDCI) [MIM:613204]. A form of congenital muscular dystrophy. Patients present at birth, or within the first few months of life, with hypotonia, muscle weakness and often with joint contractures.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
In renewing intestinal epithelium, expression of isoforms containing segment B correlates with the onset of enterocytic differentiation.
ADP-ribosylated on at least two sites of the extracellular domain in skeletal myotubes. A 70 kDa form is created by proteolytic cleavage. Cleavage is elevated during myogenic differentiation and the cleaved form enhances cell adhesion and spreading on laminin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ITGA7 antibody (ab75224)This image is courtesy of an anonymous Abreview
ab75224 staining ITGA7 (green) in Human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with methacarn and blocked with 10% serum for 20 minutes at 22°C; antigen retrieval was by heat mediation in a tris buffer. Samples were incubated with primary antibody (1/100) for 12 hours. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. 10x objective.
Immunohistochemistry (Frozen sections) - Anti-ITGA7 antibody (ab75224)This image is courtesy of an Abreview submitted by Kristin Heller.
ab75224 staining ITGA7 in Human skeletal muscle tissue by Immunohistochemistry (Frozen sections). The sections were permeabilized in Triton X-100 prior to blocking with 10% Goat serum for 1 hour at 25°C. The primary antibody was diluted 1/100 in 1xPBS/10% Goat Serum/0.1% Triton and incubated with the sample for 12 hours at 4°C. An Alexa Fluor 568®-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/250.
Simon L et al. Chronic binge alcohol consumption alters myogenic gene expression and reduces in vitro myogenic differentiation potential of myoblasts from rhesus macaques. Am J Physiol Regul Integr Comp Physiol306:R837-44 (2014).
Read more (PubMed: 24671243) »
Heller KN et al. AAV-mediated overexpression of human a7 integrin leads to histological and functional improvement in dystrophic mice. Mol Ther21:520-5 (2013).
Read more (PubMed: 23319059) »