製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to ISY1
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Recombinant fragment corresponding to amino acids 23-146 of Human ISY1 (Q9ULR0).

  • ポジティブ・コントロール
    • Human cell line U-2 OS; RT-4 cells, U-251 MG cells and Human tonsil lysates; Human stomach tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab121250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB 1/250 - 1/500.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

ターゲット情報

画像

  • ICC/IF (Immunocytochemistry/immunofluorescence) of Human cell line U-2 OS labelling ISY1 (green) with ab121250 at 1µg/ml. Cells were fixed with PFA and permeabilized with Triton X-100. Immunofluorescent staining shows positivity in nucleus but not nucleoli.

  • All lanes : Anti-ISY1 antibody (ab121250) at 1/250 dilution

    Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : Human plasma lysate
    Lane 5 : Liver lysate
    Lane 6 : Tonsil lysate
  • Immunohistochemical staining of paraffin embedded Human stomach, using 1/75 ab121250, shows moderate nuclear positivity in glandular cells.
  • Immunohistochemical staining of Human stomach shows moderate nuclear positivity in glandular cells using ab121250

参考文献

This product has been referenced in:
  • Guilgur LG  et al. Requirement for highly efficient pre-mRNA splicing during Drosophila early embryonic development. Elife 3:e02181 (2014). WB . Read more (PubMed: 24755291) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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