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Synthetic peptide conjugated to KLH derived from within residues 700 - 800 of Fruit fly (Drosophila melanogaster) ISWI, acetylated at K753.
(Peptide available as ab16064.)
|ELISA||Use at an assay dependent dilution. This antibody gave a positive result in ELISA against the immunizing peptide (ab16064). It gave a negative result in ELISA against the non-modified equivalent peptide (ab96245). This indicates that it is specific for the modified peptide.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22949616|
Recombinant ISWI (200 ng) was acetylated using 125 ng (lanes 2 and 3) of hGCN5 and a final concentration of 6
Acetylated proteins were detected by western blotting using the acetylated ISWI (K753) polyclonal antibody (ab10748), diluted 1/2500 (lanes1 and 2) or 1/500 (lanes 3 and 4) in 3% milk 0.1% T-PBS. In lanes 3 and 4, 1/5th of the reaction was loaded.
In lanes 3 and 4, 1/5th of the reaction was loaded.
Drosophila melanogaster SL-2 cells were treated for 8 days with ISWI (lane 1) or control (lane 2) dsRNAs. Total cell extracts (30