Anti-Isoleucyl tRNA synthetase 抗体 (ab31533)

製品の概要

  • 製品名Anti-Isoleucyl tRNA synthetase antibody
    Isoleucyl tRNA synthetase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Isoleucyl tRNA synthetase
  • 特異性This antibody reacts specifically with Isoleucyl tRNA synthetase protein.
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    S1074-F1264 denatured recombinant human IRS.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 145 kDa.
IP Use at an assay dependent dilution.
ICC/IF 1/1000.

ターゲット情報

  • 関連性Isoleucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of isoleucine with isoleucyl tRNA.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • FLJ20736 antibody
    • IARS antibody
    • IARS1 antibody
    • IleRS antibody
    • ILRS antibody
    • IRS antibody
    • Isoleucine tRNA ligase 1 cytoplasmic antibody
    • Isoleucine tRNA ligase antibody
    • Isoleucine tRNA synthetase antibody
    • Isoleucyl tRNA synthetase antibody
    • PRO0785 antibody
    see all

Anti-Isoleucyl tRNA synthetase antibody 画像

  • ICC/IF image of ab31533 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31533, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Isoleucyl tRNA synthetase antibody (ab31533) at 1/2000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 145 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

Anti-Isoleucyl tRNA synthetase antibody (ab31533) 使用論文

ab31533 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab31533.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"