Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528)

製品の概要

  • 製品名Isocitrate Dehydrogenase Assay Kit (Colorimetric)
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプEnzyme activity
  • 検出感度
    > 0.01 mU/well
  • 製品の概要

    Abcam's Isocitrate Dehydrogenase Assay Kit (Colorimetric) provides a convenient tool for sensitive detection of NAD(+) / NADP(+)-dependent or both IDHs in a variety of samples. The IDHs utilize isocitrate as a specific substrate leading to a proportional color development and can be easily quantified colorimetrically (? = 450 nm) with detection sensitivity as low as 0.01 mU.
    Visit our FAQs page for tips and troubleshooting.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

  • 関連性Isocitrate dehydrogenase (IDH; EC 1.1.1.41, NAD+) is an enzyme that participates in the citric acid cycle. These IDH3 isoforms catalyze the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate and CO2 while converting NAD+ to NADH. This is a two-step process, which involves oxidation of isocitrate to oxalosuccinate, followed by the decarboxylation of the beta-carboxyl group to form the ketone, alpha-ketoglutarate. Other isoforms (EC 1.1.1.42, NADP+) catalyze the same reaction, but unrelated to the citric acid cycle. It is carried out in the mitochondrion (IDH2) as well as in the cytosol and peroxisome (IDH1) and use NADP+ as a cofactor instead of NAD+.
  • 細胞内局在Cytoplasm. Peroxisome
  • 別名
    • Cytosolic NADP isocitrate dehydrogenase
    • EC=1.1.1.42
    • IDH
    • IDP
    • Isocitrate dehydrogenase [NADP] cytoplasmic
    • NADP(+) specific ICDH
    • Oxalosuccinate decarboxylase
    • PICD
    see all

アプリケーション

Our Abpromise guarantee covers the use of ab102528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Isocitrate Dehydrogenase Assay Kit (Colorimetric) 画像

  • Example of NADH and NADPH Standard Curve obtained using ab102528
  • Isocitrate Dehydrogenase detection in Bovine Liver Extraction Sample using ab102528

プロトコール

Isocitrate Dehydrogenase Assay Kit (Colorimetric) (ab102528) 使用論文

This product has been referenced in:
  • Llobet L  et al. OXPHOS xenobiotics alter adipogenic differentiation at concentrations found in human blood. Dis Model Mech N/A:N/A (2015). Functional Studies ; Human . Read more (PubMed: 26398948) »
  • Templeton N  et al. The impact of anti-apoptotic gene Bcl-2? expression on CHO central metabolism. Metab Eng 25:92-102 (2014). Read more (PubMed: 25014175) »

See all 2 Publications for this product

Product Wall

Abreviews
Good linearity for standards and easy to use. The instructions were convoluted (and actually erroneous) but that has since been corrected. Had one issue where NADP and NAD were inversely labeled (real problem for IDH assays) but new kit was sent out without issue. Worked well on both purified enzyme and cell extracts. Unfortunately the substrate concentration is not given, so if you are interested in knowing the isocitrate concentration, you will have to make your own stock (works, we did it).
Username

Dr. Jason Maynes

Verified customer

投稿 Apr 08 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry the leabeling issue caused some trouble and inconvenience. As requested, I have issued...

Read More

Thank you for bringing this to our attention. I have asked our supplying lab and our stock department to check on the kits that we currently have in stock.

The brown-capped vial should contain NADP+ and the blue-capped vial should contain NAD...

Read More

Thank you for contacting us. You may be interested in the following kits:

Citrate Synthase: ab119692
Aconitase: ab109712 or ab83459
CitrateLyase: wedo not currently have a kit available, but weyou may be interested in ourprimary ant...

Read More

1. The IDH substrate is isocitrate.

2. The substrate we provide has the optimal concentration for this assay to work. However, the exact concentration is proprietary information and cannot be given out. But if you client is facing any problem...

Read More

Thank you for contacting us and your interest in our products.

I have been checking on the suitability of the Isocitrate Dehydrogenase Colorimetric Assay Kit (ab102528)with bacterial samplesas you are hoping to use.

Although it has ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"