製品の概要

  • 製品名Isocitrate Assay Kit
  • テスト
    100 x 1 assay
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプQuantitative
  • 検出感度
    > 0.2 µg
  • 検出範囲
    0.2 µg - 5 µg
  • 全工程の試験時間
    0h 40m
  • 製品の概要

    Isocitrate Assay Kit (ab83424) provides a simple, sensitive and rapid means of quantifying isocitrate in a variety of samples. In this assay, isocitrate is oxidized with the generation of NADPH which converts a nearly colorless probe to an intensely colored species with a λmax of 450nm. This assay can detect 1 to 20 nmoles (~0.2-5 µg) of isocitrate.
    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and α-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to α-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.

  • アプリケーション適用あり: Functional Studiesmore details

法規制情報

製品の特性

  • 関連性Isocitric acid (HOOC-CHOH-CH (-COOH)-CH2-COOH) is an intermediate of the Krebs TCA cycle, positioned between citrate and alpha-keto-glutarate. It is the branch point from which the glyoxylate shunt operates in plants and lower organisms. Isocitrate is found in substantial concentrations in many fruits and vegetables as well as in foods produced from these raw materials. In the TCA cycle, isocitrate is oxidized by isocitrate dehydrogenase (IDH) to alpha-ketoglutarate with the generation of NAD(P)H. Loss of NAD-IDH has been implicated as a potential causative factor in retinitis pigmentosa.
  • 別名
    • Isocitric acid

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent dilution.

Isocitrate Assay Kit 画像

  • Isocitrate standard curve generated using this kit protocol.

プロトコール

Isocitrate Assay Kit (ab83424) 使用論文

ab83424 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I would be pleased to help answer your questions. 1. I can confirm you should be able to use these kits with blood samples. Generally serum or plasma that is obtained from blood samples is used for such purposes. Howev...

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Thank you for your enquiry regarding ab83396 and ab83424. Yes, you can use these kits with blood samples. I would suggest obtaining serum / plasma from the blood sample before proceeding with the assay. For blood plasma- “Blood plasma is prepared by...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"