Anti-Islet 1 抗体 - Neural Stem Cell Marker (ab20670)

製品の概要

  • 製品名Anti-Islet 1 antibody - Neural Stem Cell Marker
    Islet 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Islet 1 - Neural Stem Cell Marker
  • 特異性ab20670 might also detect rat and human Islet 2 protein as the immunogen used to raise this ab20670 is 86% identical to rat and human Islet 2. This has not been tested.
  • アプリケーション適用あり: IHC-FoFr, IHC-P, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Apteronotus leptorhynchus
    交差が予測される動物種: Chicken, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 300 to the C-terminus of Human Islet 1.

    (Peptide available as ab21996.)

  • ポジティブ・コントロール
    • 4 day old cultures of rat dorsal root ganglion neurons (16 day old rat embryos).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab20670 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration. PubMed: 20096094
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 2 µg/ml.
IHC-Fr 1/500.

ターゲット情報

Anti-Islet 1 antibody - Neural Stem Cell Marker 画像

  • Immunohistochemical analysis of paraffin-embedded mouse E11.5 somites labelling Islet 1 with ab20670 at 1/100 dilution, followed by BioGenex polymer-HRP reagent (undiluted according to manufacturer).

  • Dorsal root ganglion explants were dissected from 16 day-old rat embryos and cultured for 6 hours in vitro with Neurobasal Medium containing B27 supplement.     

    Nuclei stained positive for anti-Islet 1 antibody ab20670 at 2µg/ml. As would be expected, not all cells in this preparation were Islet 1-positive. Pre-incubation of ab20670 with the immunizing peptide ab21996 resulted in complete blocking of the antibody.

    Green = ab20670
    Blue = To-pro-3 nuclear stain

    The level of magnification is different in each image.    

  • ab20670 detected Islet 1 in the nucleus of frozen sections of E11.5 mouse embryonic brain using 1 ug/ml of antibody. Brighter staining may be achieved using a greater concentration of antibody, e.g. 2.5 or 5 ug/ml.
  • ab20670 2µg/ml staining ISLET1 in human pancreas using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and weak cytoplasmic staining primarily in the pancreatic islet.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Islet 1 antibody - Neural Stem Cell Marker (ab20670) 使用論文

This product has been referenced in:
  • Nishi K  et al. Nardilysin Is Required for Maintaining Pancreatic ß-Cell Function. Diabetes 65:3015-27 (2016). Read more (PubMed: 27385158) »
  • Beaudet MJ  et al. High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord. Sci Rep 5:16763 (2015). Read more (PubMed: 26577180) »

See all 21 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (Differentiated mES cell)
Permeabilization Yes - 0.2% Triton X
Specification Differentiated mES cell
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Nov 23 2016

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated
Sample Spotted Catshark (Scyliorhinus canicula) Tissue sections (brain cells)
Specification brain cells
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Liam Rasch

Verified customer

投稿 Nov 05 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification Dorsal Root Ganglia (DRG) neurons
Fixative Paraformaldehyde
Permeabilization Yes - tween 20
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Oct 18 2012

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Sep 28 2012

Thank you for your message.

I am pleased the results have improved. In order to help further, I would appreciate if you are able to send an attached image which will help me to assess the results.

Thank you for your continued coop...

Read More

Thank you for taking the time to complete our questionnaire and provide further information.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quali...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable...

Read More

Thank you for contacting Abcam.

Please find attached the Certificate of Compliance for ab20670, lot #*.

If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult heart)
Specification Adult heart
Fixative Formaldehyde
Permeabilization Yes - 0.5% Tx100 in PBS
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jun 19 2012

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"