製品の概要

  • 製品名Anti-Islet 1 antibody [EP4182]
    Islet 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP4182] to Islet 1
  • 特異性86% identities with Islet 2
  • アプリケーション適用あり: ICC/IF, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Islet 1 aa 250 to the C-terminus.
    Database link: P61371

  • ポジティブ・コントロール
    • WB: K562, HeLa, Jurkat and SH-SY5Y cell lysates IHC-Fr: Chicken hindbrain ICC/IF: U-87 MG cell line.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-Islet 1 antibody (Alexa Fluor® 594) [EP4182] (ab203404)

    Anti-Islet 1 antibody (Alexa Fluor® 555) [EP4182] (ab203405)

    Anti-Islet 1 antibody (Alexa Fluor® 405) [EP4182] (ab202297)

    Anti-Islet 1 antibody (Alexa Fluor® 568) [EP4182] (ab203406)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.

For unpurified use at 1/50 - 1/100.

WB 1/10000 - 1/50000. Predicted molecular weight: 39 kDa.
IP 1/10 - 1/100.
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds to one of the cis-acting domain of the insulin gene enhancer.
  • 組織特異性Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • 配列類似性Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

Anti-Islet 1 antibody [EP4182] 画像

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + RAW264.7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (human neuroblastoma) cells labelling Islet 1 with purified ab109517 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry analysis of SH-SY5Y(human neuroblastoma) cells labeling Islet 1 with purified ab109517 at 1/50 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/10000 dilution (purified) + UMNSAH/DF-1 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • IHC-Fr image of anti-Islet 1 staining with unpurified ab109517 on tissue sections from chicken hindbrain. The sections were blocked with 3% BSA for 1 hour at 4°C, before incubation with unpurified ab109517 (1/1000 dilution) for 16 hours at 4°C. The secondary was an Alexa-Fluor 568 conjugated goat anti-rabbit polyclonal, used at a 1/1000 dilution.

    See Abreview

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunocytochemistry/Immunofluorescence analysis of U-87 MG cells labelling Islet 1 with unpurified ab109517.

  • Anti-Islet 1 antibody [EP4182] (ab109517) at 1/50000 dilution (purified) + K562 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size : 39 kDa

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

  • All lanes : Anti-Islet 1 antibody [EP4182] (ab109517) at 1/10000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 39 kDa
  • ab109517 (purified) at 1/30 immunoprecipitating Islet 1 in Jurkat whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP secondary antibody (ab131366) (1/1,500) was used as the secondary antibody. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • ab109517 (unpurified) immunoprecipitating Islet 1 in Jurkat whole cell lysate.

Anti-Islet 1 antibody [EP4182] (ab109517) 使用論文

This product has been referenced in:
  • Nasif S  et al. Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood. Proc Natl Acad Sci U S A 112:E1861-70 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25825735) »
  • Zhang Q  et al. ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex. Mol Cancer 13:181 (2014). WB ; Human . Read more (PubMed: 25070240) »

See all 3 Publications for this product

Product Wall

Application ChIP
Detection step Real-time PCR
Sample Mouse Cell lysate - nuclear (Min6)
Specification Min6
Negative control Negative control for Isl-1 antibody: known locus without enrichment Positive control for IP: Antibody for another factor that is not enriched at that locus
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1.1% Formaldehyed
Positive control Positive control for Isl-1 antibody: known locus of enrichment Positive control for IP: Antibody for another factor also enriched at that locus
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投稿 Mar 24 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification Human pluripotent stem cell derived cardiomyocyte
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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投稿 Oct 16 2014

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Sample Chicken Tissue sections (hindbrain section)
Specification hindbrain section
Permeabilization Yes - Triton-X
Fixative Paraformaldehyde
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投稿 Oct 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"