製品の概要

  • 製品名
    Anti-Islet 1 antibody [1H9]
    Islet 1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1H9] to Islet 1
  • 由来種
    Mouse
  • 特異性
    In western blot, we observe a specific band at ~40kDa which is not seen in KO cell lines. A cross-reactive band was observed in the wild-type and knockout cells. The additional band below this band of interest is seen at ~38kDa in both the WT and KO cells. We are unsure as to the identity of these extra bands.
  • アプリケーション
    適用あり: Flow Cyt, WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment corresponding to Human Islet 1 aa 150-349. Purified recombinant fragment of Human Islet 1 expressed in E. Coli
    Database link: P61371

  • ポジティブ・コントロール
    • Transfected HEK293 cell lysate. Human lung cancer tissue and cervical carcinoma tissue.
  • 特記事項

    This product was changed from ascites to supernatant. Lot no’s high than GR141006-29 are from Tissue Culture Supernatant

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/50 - 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 39 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
ICC/IF 1/200 - 1/1000.

ターゲット情報

  • 機能
    Binds to one of the cis-acting domain of the insulin gene enhancer.
  • 組織特異性
    Expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain.
  • 配列類似性
    Contains 1 homeobox DNA-binding domain.
    Contains 2 LIM zinc-binding domains.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Insulin gene enhancer protein ISL 1 antibody
    • Insulin gene enhancer protein ISL-1 antibody
    • Insulin related protein antibody
    • ISL 1 antibody
    • ISL LIM homeobox 1 antibody
    • ISL1 antibody
    • ISL1 transcription factor LIM homeodomain antibody
    • ISL1 transcription factor, LIM/homeodomain (islet 1) antibody
    • ISL1 transcription factor, LIM/homeodomain antibody
    • ISL1_HUMAN antibody
    • Islet-1 antibody
    • Islet1 antibody
    see all

画像

  • All lanes : Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : ISL1 (Islet 1) knockout HAP1 whole cell lysate
    Lane 3 : SH-SY5Y whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 39 kDa
    Observed band size: 40 kDa (why is the actual band size different from the predicted?)



    Lanes 1 - 4: Merged signal (red and green). Green - ab86472 observed at 40 kDa. Red - loading control, ab176560, observed at 50 kDa.

    ab86472 was shown to recognize Islet 1 in wild-type HAP1 cells as signal was lost at the expected MW in Islet 1 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and Islet 1 knockout samples were subjected to SDS-PAGE. ab86472 and ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Anti-Islet 1 antibody [1H9] (ab86472) at 1/500 dilution + HEK293 cells transfected with full length Islet 1 (aa1-349)-hIgGFc

    Predicted band size: 39 kDa



    Note: The molecular weight is larger than expected, as the recombinant protein is not the endogenouse protein, but rather the fc-fusion protein

  • Confocal immunofluorescence analysis of HEK293 cells trasfected with full-length Islet 1-hIgGFc using ab86472 at 1/150 dilution (green). Blue: DRAQ5 fluorescent DNA dye.

  • ab86472 at a 1/200 dilution staining Islet 1 in human lung cancer tissue. DAB staining shows nuclear localisation.
  • ab86472 at a 1/200 dilution staining Islet 1 in human cervical carcinoma tissue. DAB staining shows nuclear localization.
  • Overlay histogram showing SH-SY5Y cells stained with ab86472 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86472, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

参考文献

This product has been referenced in:
  • Hernandez I  et al. Short-term hypoxia improves early cardiac progenitor cell function in vitro. Am J Stem Cells 7:1-17 (2018). Flow Cyt ; Human . Read more (PubMed: 29531855) »
  • Zhang Q  et al. ISL-1 is overexpressed in non-Hodgkin lymphoma and promotes lymphoma cell proliferation by forming a p-STAT3/p-c-Jun/ISL-1 complex. Mol Cancer 13:181 (2014). IHC-P ; Human . Read more (PubMed: 25070240) »

See all 6 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 37°C
Sample
Dog Tissue sections (Heart)
Specification
Heart
Permeabilization
Yes - 0.5% Tween80 PBS wash
Fixative
Paraformaldehyde
Username

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Verified customer

投稿 Oct 13 2014

Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it...

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Thank you for contacting Abcam regarding ab86472. I am sorry that you are experiencing difficulties with some lots of this antibody in IHC. I have checked our current stock and currently we have a new lot available. I would be happy to send this as...

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Vielen Dank fuer Ihre email. Es tut mir sehr leid zu hoeren, dass das aktuelle Lot fuer ab86472 Ihnen Probleme bereitet. Eine Ersatzlieferung waere auf jeden Fall moeglich, falls der Antikoerper in getesteten Anwendungen (ELISA, ICC/IF, IHC-P, W...

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