Anti-IRX2 抗体 (ab72975)
Key features and details
- Mouse polyclonal to IRX2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-IRX2 antibody
IRX2 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to IRX2 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human IRX2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Hela cells and IRX2 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72975の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | ||
ICC/IF |
特記事項 |
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Protein A purify ab72975 before starting ICC/IF procedure.
WB: 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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配列類似性
Belongs to the TALE/IRO homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 153572 Human
- Omim: 606198 Human
- SwissProt: Q9BZI1 Human
- Unigene: 282089 Human
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別名
- Homeodomain protein IRXA2 antibody
- Iroquois homeobox 2 antibody
- Iroquois homeobox protein 2 antibody
see all
画像
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All lanes : Anti-IRX2 antibody (ab72975) at 1/500 dilution
Lane 1 : IRX2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa -
HeLa cells with Protein A purified ab72975 at 10ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab72975 は 2 報の論文で使用されています。
- Liu T et al. Lineage conversion of mouse fibroblasts to pancreatic a-cells. Exp Mol Med 49:e350 (2017). ICC/IF . PubMed: 28665920
- Liu T et al. IRX2-mediated upregulation of MMP-9 and VEGF in a PI3K/AKT-dependent manner. Mol Med Rep 12:4346-4351 (2015). PubMed: 26062523