製品の概要

  • 製品名Anti-IRS4 antibody [EP907Y]
    IRS4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP907Y] to IRS4
  • アプリケーション適用あり: IHC-P, WB, IP, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues from the C terminus of human IRS4.

  • ポジティブ・コントロール
    • Hek293 cell lysate and Human kidney tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
WB 1/5000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
IP 1/20.
Flow Cyt 1/20 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性IRS4 (Insulin receptor substrate 4) is a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signaling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation.
  • 細胞内局在Cell membrane; Peripheral membrane protein; Cytoplasmic side.
  • 参照データベース
  • 別名
    • 160 kDa phosphotyrosine protein antibody
    • Insulin receptor substrate 4 antibody
    • IRS 4 antibody
    • PY160 antibody

Anti-IRS4 antibody [EP907Y] 画像

  • Anti-IRS4 antibody [EP907Y] (ab52622) at 1/5000 dilution + Hek293 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 133 kDa
    Observed band size : 133 kDa
  • ab52622, at a 1/50 dilution staining Human IRS4 in kidney using Immunohistochemistry, Paraffin embedded tissue.
  • Overlay histogram showing HEK293 cells stained with ab52622 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52622, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-IRS4 antibody [EP907Y] (ab52622) 使用論文

This product has been referenced in:
  • Homma Y  et al. Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. J Biol Chem 289:26302-13 (2014). Read more (PubMed: 25100728) »
  • Chai B  et al. A novel transcript is up-regulated by fasting in the hypothalamus and enhances insulin signaling. J Neuroendocrinol : (2012). Read more (PubMed: 22935015) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"