製品の概要

  • 製品名Anti-IRS2 antibody [EP976Y]
    IRS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP976Y] to IRS2
  • アプリケーション適用あり: WB, IP, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues surrounding Ser1203 of human IRS2.

  • ポジティブ・コントロール
    • 293 cell lysate; human kidney tissue. IF/ICC: SKNSH cell line.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab52606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000. Detects a band of approximately 185 kDa (predicted molecular weight: 137 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能May mediate the control of various cellular processes by insulin.
    • 配列類似性Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm > cytosol.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP976Y] 画像

    • ab52606 stained SKNSH cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52606 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Anti-IRS2 antibody [EP976Y] (ab52606) at 1/100000 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 185 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of paraffin-embedded human kidney using ab52606 at a dilution of 1/100-1/250.

    Anti-IRS2 antibody [EP976Y] (ab52606) 使用論文

    ab52606 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"