製品の概要

  • 製品名Anti-IRS2 antibody [EP903Y]
    IRS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP903Y] to IRS2
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human IRS2.

  • ポジティブ・コントロール
    • 293 cell lysate + insulin; human cervical carcinoma tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab46811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100000. Predicted molecular weight: 137 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能May mediate the control of various cellular processes by insulin.
    • 配列類似性Contains 1 IRS-type PTB domain.
      Contains 1 PH domain.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Cytoplasm > cytosol.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Insulin receptor substrate 2 antibody
      • IRS 2 antibody
      • IRS-2 antibody
      • Irs2 antibody
      • IRS2_HUMAN antibody
      see all

    Anti-IRS2 antibody [EP903Y] 画像

    • Anti-IRS2 antibody [EP903Y] (ab46811) at 1/100000 dilution + 293 cell lysate at 10ug/lane, plus insulin

      Secondary
      Goat-anti rabbit, HRP labelled, at 1/2000 dilution

      Predicted band size : 137 kDa
      Observed band size : 160 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 50 kDa (possible non-specific binding).
      The 50 kDa band is most likely the heavy chain. This appears when using human tissue or samples which may contain traces of blood. There are secondary antibodies such as Tru-Blot that can eliminate this heavy chain band.
    • This image shows human cervical carcinoma stained with ab46811 at 1/50 dilution.

    Anti-IRS2 antibody [EP903Y] (ab46811) 使用論文

    This product has been referenced in:
    • Lopez R  et al. Hyperglycemia enhances the proliferation of non-tumorigenic and malignant mammary epithelial cells through increased leptin/IGF1R signaling and activation of AKT/mTOR. PLoS One 8:e79708 (2013). WB ; Human . Read more (PubMed: 24260287) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab46811.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"