製品の概要

  • 製品名
    Anti-IRS1 (phospho S307) antibody
    IRS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IRS1 (phospho S307)
  • 特異性
    Recognises a 180kDa band in 293 cells stimulated with anisomysin after serum starvation.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig
  • 免疫原

    Synthetic phospho peptide: GLTRRSRTESpITATSPASM conjugated to KLH, corresponding to amino acids 298-316 of Human IRS 1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Detects a band of approximately 180 kDa (predicted molecular weight: 131 kDa).

ターゲット情報

  • 機能
    May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.
  • 関連疾患
    Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
  • 配列類似性
    Contains 1 IRS-type PTB domain.
    Contains 1 PH domain.
  • 翻訳後修飾
    Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor.
    Phosphorylation of Tyr-896 is required for GRB2-binding.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HIRS 1 antibody
    • HIRS1 antibody
    • Insulin receptor substrate 1 antibody
    • IRS 1 antibody
    • IRS-1 antibody
    • IRS1 antibody
    • IRS1_HUMAN antibody
    • OTTHUMP00000164234 antibody
    see all

画像

  • All lanes : Anti-IRS1 (phospho S307) antibody (ab1194) at 1/250 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min.

    Lysates/proteins at 25 µg per lane.

    Secondary
    IRDye800 Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 131 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

This product has been referenced in:

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