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Our Abpromise guarantee covers the use of ab69494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21102437|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 21-46 kDa). please refer to databases and the literature for the expected molecular weights of species orthologues.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Paraffin-embedded human thymus tissue stained for IRGM using ab69494 at 5 µg/ml in immunohistochemical analysis.