製品の概要
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製品名Anti-IRGM antibody [403CT8.5.5]
IRGM 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [403CT8.5.5] to IRGM
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アプリケーション適用あり: WBmore details
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種交差性交差種: Mouse
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免疫原
Full-length recombinant mouse IRGM protein.
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ポジティブ･コントロール
- This antibody gave a positive signal in mouse brain and mouse heart tissue lysates.
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特記事項
This antibody clone is manufactured by Abcam.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度Concentrated Culture Supernatant
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ポリ/モノモノクローナル
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クローン名403CT8.5.5
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ミエローマSp2/0
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アイソタイプIgM
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab63230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/100. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Block using 3% milk. |
ターゲット情報
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機能Putative GTPase which is required for clearance of acute protozoan and bacterial infections. Functions in innate immune response probably through regulation of autophagy. May regulate proinflammatory cytokine production and prevent endotoxemia upon infection. May also play a role in macrophages adhesion and motility.
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組織特異性Widely expressed (at protein level). Expressed in several tissues including colon, small bowel and peripheral blood leukocytes.
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関連疾患Defects in IRGM are the cause of susceptibility to inflammatory bowel disease type 19 (IBD19) [MIM:612278]. A chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
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配列類似性Belongs to the interferon-inducible GTPase family.
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細胞内局在Golgi apparatus membrane. Cell membrane. Cytoplasmic vesicle > phagosome membrane. Cytoplasmic vesicle > autophagosome membrane. Cell projection > phagocytic cup. Behaves like an integral membrane protein (By similarity). Recruited to the plasma membrane around forming phagocytic cups, it remains associated with maturing autophagosomes (By similarity). Preferentially associated with cis- and medial-Golgi.
- Information by UniProt
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参照データベース
- Entrez Gene: 15944 Mouse
- SwissProt: Q60766 Mouse
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別名
- IFI1 antibody
- Iigp3 antibody
- Iipg3 antibody
see all
Anti-IRGM antibody [403CT8.5.5] 画像
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![Western blot - Anti-IRGM antibody [403CT8.5.5] (ab63230)](http://a.abcam.com/ps/datasheet/images/63/ab63230/IRGM-Primary-antibodies-ab63230-2.jpg)
Western blot - Anti-IRGM antibody [403CT8.5.5] (ab63230)All lanes : Anti-IRGM antibody [403CT8.5.5] (ab63230) at 1/100 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 47 kDa
Observed band size : 45,47 kDa (why is the actual band size different from the predicted?)
Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
プロトコール
Anti-IRGM antibody [403CT8.5.5] (ab63230) 使用論文
ab63230 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"