製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IRF8
  • 特異性
    ab28696 recognises Interferon regulatory factor 8 (IRF8).
  • アプリケーション
    適用あり: WB, IHC-P, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide derived from a region between amino acids 43-92 of human ICSBP. (Note: The exact amino acid sequence is proprietary).

  • ポジティブ・コントロール
    • Jurkat cell lysate; Human kidney slide This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab28696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system.
  • 組織特異性
    Predominantly in lymphoid tissues.
  • 配列類似性
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻訳後修飾
    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • H ICSBP antibody
    • H-ICSBP antibody
    • HGNC:5358 antibody
    • HICSBP antibody
    • ICSBP 1 antibody
    • ICSBP antibody
    • ICSBP1 antibody
    • Interferon consensus sequence binding protein 1 antibody
    • Interferon consensus sequence binding protein antibody
    • Interferon consensus sequence-binding protein antibody
    • Interferon regulatory factor 8 antibody
    • IRF 8 antibody
    • IRF-8 antibody
    • Irf8 antibody
    • IRF8_HUMAN antibody
    • MYLS antibody
    see all

画像

  • ICC/IF image of ab28696 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab28696 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-IRF8 antibody (ab28696) at 1.25 µg/ml + Jurkat cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG

    Predicted band size : 47 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Western blot with ab28696 at 1.25µg/ml using HRP conjugated anti-Rabbit IgG diluted in 1/50000 - 1/100000 as secondary antibody and jurkat cell lysate at a gel concentration of 12%.
  • Immunohistochemistry of Human Intestine tissue (ab28696)

  • Immunohistochemistry of Human Liver cell lysate tissue (ab28696)

参考文献

This product has been referenced in:
  • McEwan WA  et al. Intracellular antibody-bound pathogens stimulate immune signaling via the Fc receptor TRIM21. Nat Immunol 14:327-36 (2013). Read more (PubMed: 23455675) »
  • Woo AJ  et al. Developmental differences in IFN signaling affect GATA1s-induced megakaryocyte hyperproliferation. J Clin Invest N/A:N/A (2013). IHC-P . Read more (PubMed: 23863621) »

See all 3 Publications for this product

レビューと Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spleen)
Permeabilization
Yes - Acetone
Specification
Spleen
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

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投稿 Jan 06 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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