製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IRF7
  • 特異性
    This antibody reacts with IRF7
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7

  • ポジティブ・コントロール
    • 293 whole cell lysate and mouse spleen tissue HepG2 cells. Jurkat whole cells extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human IRF7 peptide (ab92594).
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
  • 組織特異性
    Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
  • 配列類似性
    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻訳後修飾
    In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
    TRAF6-mediated ubiquitination is required for IRF7 activation.
  • 細胞内局在
    Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • IMD39 antibody
    • Interferon regulatory factor 7 antibody
    • Interferon regulatory factor 7H antibody
    • IRF 7 antibody
    • IRF 7A antibody
    • IRF 7H antibody
    • IRF-7 antibody
    • IRF7 antibody
    • IRF7_HUMAN antibody
    • IRF7A antibody
    • IRF7B antibody
    • IRF7C antibody
    • IRF7H antibody
    see all

画像

  • ICC/IF image of ab62505 stained HepG2 cells (ab7900). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62505, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of IRF7 in Mouse Spleen cells using ab62505 at 20 ug/ml.

  • Lane 1 : Anti-IRF7 antibody (ab62505) at 0.5 µg/ml
    Lane 2 : Anti-IRF7 antibody (ab62505) at 1 µg/ml
    Lane 3 : Anti-IRF7 antibody (ab62505) at 2 µg/ml

    Lane 1 : 293 whole cell lysate
    Lane 2 : 293 whole cell lysate
    Lane 3 : 293 whole cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 51 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of IRF7 in paraffin embedded mouse spleen tissue section with ab62505 antibody at 5 µg/ml.
  • IRF7 was immunoprecipitated using 0.5mg Jurkat whole cell extract (ab7899), 5µg of Rabbit polyclonal to IRF7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab62505.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 49kDa: IRF7; non specific - 40kDa: We are unsure as to the identity of this extra band.

参考文献

This product has been referenced in:
  • Katlinskaya YV  et al. Type I Interferons Control Proliferation and Function of the Intestinal Epithelium. Mol Cell Biol 36:1124-35 (2016). Read more (PubMed: 26811327) »
  • Yu Q  et al. Type I interferons mediate pancreatic toxicities of PERK inhibition. Proc Natl Acad Sci U S A 112:15420-5 (2015). Read more (PubMed: 26627716) »

See all 7 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Pig Cell lysate - whole cell (Modified SK6 (Swine Kidney) cells)
Gel Running Conditions
Non-reduced Denaturing (12% gel)
Loading amount
75000 cells
Treatment
1ug Poly I:C
Specification
Modified SK6 (Swine Kidney) cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Hanah Georges

Verified customer

投稿 Jul 14 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - PBS with 3% Triton
Specification
Mammary gland tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 04 2015

Application
Western blot
Sample
Dog Cell lysate - whole cell (CPEK)
Gel Running Conditions
Reduced Denaturing (4-20% polyacrylamide)
Loading amount
20 µg
Treatment
stim-stimulated PolyADT
Specification
CPEK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Apr 16 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample
Mouse Tissue sections (Liver, P5)
Specification
Liver, P5
Permeabilization
Yes - Triton 0,05%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 07 2014

Thank you for contacting us.

Regarding ab109255, the immunogen is within aa 200-250 and is able to detect isoforms A, B and D.


For ab62505:

The immunogen does not span a phosphorylated region (it is N-terminal of the pho...

Read More

Thank you for your call today and for letting us know about the trouble with ab62505.

As we discussed, I'm sending a free of charge vial of ab109255 on the order ***, which should arrive tomorrow. Please keep me updated about the results with...

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Thank you for contacting us.

The IRF7 protein according to Uniprot (http://www.uniprot.org/uniprot/Q92985) have isoform molecular weight 54, 51, 18 and 55 kDa. Please note, the molecular weight for unprocessed protein (predicted) is based on...

Read More

I am currently working on this inquiry. I will get back to you soon.

Many thanks for having patience!

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (bone marrow derived-macrophages)
Loading amount
30 µg
Specification
bone marrow derived-macrophages
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Dec 01 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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