製品の概要
- 製品名Anti-IRF7 antibody
IRF7 一次抗体 製品一覧 - 製品の詳細Rabbit polyclonal to IRF7
- 特異性This antibody reacts with IRF7
- アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
- 種交差性交差種: Mouse, Rat, Human
- 免疫原
A synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human IRF7
- ポジティブ･コントロール
- 293 whole cell lysate and mouse spleen tissue HepG2 cells. Jurkat whole cells extract.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
- バッファーPreservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... - 精製度Immunogen affinity purified
- 特記事項（精製）IRF7 Antibody is affinity chromatography purified via peptide column.
- ポリ/モノポリクローナル
- アイソタイプIgG
- 研究分野
関連製品
- Compatible Secondaries
- Immunizing Peptide (Blocking)
- Positive Controls
- Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab62505 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 54 kDa).Can be blocked with Human IRF7 peptide (ab92594). | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. |
ターゲット情報
- 機能Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) in IFN promoters and in the Q promoter (Qp) of EBV nuclear antigen 1 (EBNA1). Functions as a molecular switch for antiviral activity. Activated by phosphorylation in response to infection. Activation leads to nuclear retention, DNA binding, and derepression of transactivation ability.
- 組織特異性Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
- 配列類似性Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. - 翻訳後修飾In response to a viral infection, phosphorylated on the C-terminal serine cluster. Phosphorylation, and subsequent activation is inhibited by vaccinia virus protein E3.
TRAF6-mediated ubiquitination is required for IRF7 activation. - 細胞内局在Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus.
- Information by UniProt
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参照データベース
- Entrez Gene: 3665 Human
- Entrez Gene: 54123 Mouse
- Entrez Gene: 293624 Rat
- Omim: 605047 Human
- SwissProt: Q92985 Human
- SwissProt: P70434 Mouse
- Unigene: 166120 Human
- Unigene: 3233 Mouse
see all -
別名
- IMD39 antibody
- Interferon regulatory factor 7 antibody
- Interferon regulatory factor 7H antibody
see all
Anti-IRF7 antibody 画像
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Immunocytochemistry/ Immunofluorescence - Anti-IRF7 antibody (ab62505)ICC/IF image of ab62505 stained HepG2 cells (ab7900). The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum (ab7481) / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62505, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunocytochemistry/ Immunofluorescence - Anti-IRF7 antibody (ab62505)Immunofluorescence of IRF7 in Mouse Spleen cells using ab62505 at 20 ug/ml. -
Western blot - Anti-IRF7 antibody (ab62505)Lane 1 : Anti-IRF7 antibody (ab62505) at 0.5 µg/ml
Lane 2 : Anti-IRF7 antibody (ab62505) at 1 µg/ml
Lane 3 : Anti-IRF7 antibody (ab62505) at 2 µg/ml
Lane 1 : 293 whole cell lysate
Lane 2 : 293 whole cell lysate
Lane 3 : 293 whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 54 kDa
Observed band size : 51 kDa (why is the actual band size different from the predicted?) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRF7 antibody (ab62505)Immunohistochemistry of IRF7 in paraffin embedded mouse spleen tissue section with ab62505 antibody at 5 µg/ml. -
Immunoprecipitation - Anti-IRF7 antibody (ab62505)IRF7 was immunoprecipitated using 0.5mg Jurkat whole cell extract (ab7899), 5µg of Rabbit polyclonal to IRF7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab62505.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 49kDa: IRF7; non specific - 40kDa: We are unsure as to the identity of this extra band.
Anti-IRF7 antibody (ab62505) 使用論文
This product has been referenced in:
- Kropp KA et al. A temporal gate for viral enhancers to co-opt Toll-like-receptor transcriptional activation pathways upon acute infection. PLoS Pathog 11:e1004737 (2015). ChIP . Read more (PubMed: 25856589) »
- Dupuis-Maurin V et al. Overexpression of the transcription factor Sp1 activates the OAS-RNAse L-RIG-I pathway. PLoS One 10:e0118551 (2015). WB . Read more (PubMed: 25738304) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"