Anti-IRF6 抗体 [2A12] (ab123880)

製品の概要

  • 製品名Anti-IRF6 antibody [2A12]
    IRF6 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [2A12] to IRF6
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length Human IRF6 protein (NP_006138) produced in HEK293 cell.

  • ポジティブ・コントロール
  • 特記事項Dilute in PBS (pH 7.3) before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 53 kDa.
IHC-P 1/150.
Flow Cyt 1/100. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

ターゲット情報

  • 機能Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation.
  • 組織特異性Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas.
  • 関連疾患Defects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
    Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.
  • 配列類似性Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻訳後修飾Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation.
  • 細胞内局在Nucleus. Cytoplasm. Translocates to nucleus in response to an activating signal.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interferon regulatory factor 6 antibody
    • IRF 6 antibody
    • IRF-6 antibody
    • IRF6 antibody
    • IRF6_HUMAN antibody
    • LPS antibody
    • OFC 6 antibody
    • OFC6 antibody
    • OTTHUMP00000034677 antibody
    • OTTHUMP00000034678 antibody
    • PIT antibody
    • PPS antibody
    • PPS1 antibody
    • VWS antibody
    • VWS1 antibody
    see all

Anti-IRF6 antibody [2A12] 画像

  • All lanes : Anti-IRF6 antibody [2A12] (ab123880) at 1/2000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 53 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC201579)
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human colon tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded Human kidney tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded carcinoma of Human lung tissue by Immunohistochemistry.
  • ab123880, at a 1/150 dilution, staining IRF6 in paraffin embedded adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
  • ab123880, at a 1/100 dilution, staining IRF6 in COS7 cells transiently transfected using pCMV6-ENTRY IRF6, by Immunofluorescence.
  • ab123880, at a 1/100 dilution, staining IRF6 in HEK293T cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue), by Flow Cytometry.

Anti-IRF6 antibody [2A12] (ab123880) 使用論文

ab123880 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Granular Cell Layer)
Specification Granular Cell Layer
Fixative Formaldehyde
Permeabilization Yes - 0.5% Triton X-100
Blocking step Serum, plus monovalent Fab Fragment as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 May 18 2012

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