Anti-IRF6 抗体 [OTI2A12] (ab123880)
Key features and details
- Mouse monoclonal [OTI2A12] to IRF6
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human, African green monkey, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-IRF6 antibody [OTI2A12]
IRF6 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI2A12] to IRF6 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human, African green monkey, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human IRF6. Produced in HEK293 cell (NP_006138)
Database link: O14896 -
ポジティブ・コントロール
- WB: HEK293T cells transfected with pCMV6-ENTRY IRF6 cDNA IHC-P: adenocarcinoma of Human colon tissue, Human kidney tissue, carcinoma of Human lung tissue, adenocarcinoma of Human endometrium tissue ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY IRF6 Flow Cyt (Intra): HEK293T cells transfected with pCMV6-ENTRY IRF6 overexpress plasmid
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, PBS -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant by affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
OTI2A12 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123880の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/100.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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WB |
1/2000. Predicted molecular weight: 53 kDa.
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IHC-P |
1/150.
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ICC/IF | (1) |
1/100.
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特記事項 |
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Flow Cyt (Intra)
1/100. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
WB
1/2000. Predicted molecular weight: 53 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
ターゲット情報
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機能
Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development (By similarity). Plays a role in regulating mammary epithelial cell proliferation. -
組織特異性
Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas. -
関連疾患
Defects in IRF6 are a cause of van der Woude syndrome (VWS) [MIM:119300]; also known as lip-pit syndrome (LPS). It is an autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate. Penetrance is incomplete. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Defects in IRF6 are the cause of popliteal pterygium syndrome (PPS) [MIM:119500]. PPS is an autosomal dominant developmental disorder characterized by cleft lip and/or cleft palate, and skin and genital anomalies. Penetrance is incomplete and expressivity is variable. It shows orofacial phenotypic similarities with van der Woude syndrome. Van der Woude and popliteal pterygium syndrome are allelic disorders.
Genetic variation in IRF6 is associated with non-syndromic orofacial cleft type 6 (OFC6) [MIM:608864]; also called non-syndromic cleft lip with or without cleft palate 6. Non-syndromic orofacial cleft is a common birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum. -
配列類似性
Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻訳後修飾
Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation. -
細胞内局在
Nucleus. Cytoplasm. Translocates to nucleus in response to an activating signal. - Information by UniProt
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参照データベース
- Entrez Gene: 3664 Human
- Omim: 607199 Human
- SwissProt: O14896 Human
- Unigene: 719361 Human
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別名
- Interferon regulatory factor 6 antibody
- IRF 6 antibody
- IRF-6 antibody
see all
画像
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All lanes : Anti-IRF6 antibody [OTI2A12] (ab123880) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY IRF6 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 53 kDa -
Paraffin-embedded human colon adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung carcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for IRF6 using ab123880 at 1/150 dilution in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected using pCMV6-ENTRY IRF6 stained for IRF6 (green) in ICC/IF.
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Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with either pCMV6-ENTRY IRF6 overexpress plasmid (Red), or empty vector control plasmid (Blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab123880 は 2 報の論文で使用されています。
- Ma X et al. Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma. Cancer Cell Int 21:246 (2021). PubMed: 33941190
- Artigas-Jerónimo S et al. A Novel Combined Scientific and Artistic Approach for the Advanced Characterization of Interactomes: The Akirin/Subolesin Model. Vaccines (Basel) 8:N/A (2020). PubMed: 32046307