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RabMAb

Anti-IRF5 抗体 [EPR6094] (ab124792)

製品の概要

  • 製品名
    Anti-IRF5 antibody [EPR6094]
    IRF5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6094] to IRF5
  • アプリケーション
    適用あり: IHC-Fr, WB, Flow Cytmore details
    適用なし: ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human IRF5 (Q13568).

  • ポジティブ・コントロール
    • U937, Ramos and THP-1 cell lysates, U937 cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-IRF5 antibody (Alexa Fluor® 488) [EPR6094] (ab193245)

    Anti-IRF5 antibody (Alexa Fluor® 647) [EPR6094] (ab192983)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 56 kDa).Can be blocked with IRF5 peptide (ab174256).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 関連疾患
      Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) [MIM:612245]. IBD14 is a chronic, relapsing inflammation of the gastrointestinal tract with a complex etiology. It is subdivided into Crohn disease and ulcerative colitis phenotypes. Crohn disease may affect any part of the gastrointestinal tract from the mouth to the anus, but most frequently it involves the terminal ileum and colon. Bowel inflammation is transmural and discontinuous; it may contain granulomas or be associated with intestinal or perianal fistulas. In contrast, in ulcerative colitis, the inflammation is continuous and limited to rectal and colonic mucosal layers; fistulas and granulomas are not observed. Both diseases include extraintestinal inflammation of the skin, eyes, or joints.
      Genetic variations in IRF5 are associated with susceptibility to systemic lupus erythematosus type 10 (SLEB10) [MIM:612251]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
      Genetic variations in IRF5 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures.
    • 配列類似性
      Belongs to the IRF family.
      Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Interferon regulatory factor 5 antibody
      • Interferon regulatory factor 5 bone marrow variant antibody
      • IRF 5 antibody
      • IRF-5 antibody
      • Irf5 antibody
      • IRF5_HUMAN antibody
      • SLEB10 antibody
      see all

    画像

    • All lanes : Anti-IRF5 antibody [EPR6094] (ab124792) at 1/1000 dilution

      Lane 1 : U937 cell lysates
      Lane 2 : Ramos cell lysates
      Lane 3 : THP-1 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 56 kDa
    • Flow cytometric analysis of permeabilized U937 cells using ab124792 at 1/100 dilution (red) or a rabbit IgG (negative) (green).
    • Flow cytometry analysis of Plasmacytoid dendritic cells, staining IRF5 with ab124792.

      Cells were fixed in paraformaldehyde and fixed in saponin. The sample was incubated with the primary antibody (1/100 in 2% human serum + 0.5 mM EDTA) for 20 minutes at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/100) was used as the secondary antibody.

      Gating Strategy: FSC/SSC lymphocytes and then FSC-A/FSC-H singlets

      See Abreview

    参考文献

    This product has been referenced in:
    • Li D  et al. Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality. Sci Rep 6:31002 (2016). Read more (PubMed: 27481535) »
    • Bergstrøm B  et al. TLR8 Senses Staphylococcus aureus RNA in Human Primary Monocytes and Macrophages and Induces IFN-ß Production via a TAK1-IKKß-IRF5 Signaling Pathway. J Immunol 195:1100-11 (2015). IF ; Human . Read more (PubMed: 26085680) »

    See all 2 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Human Cell (pDC)
    Specification
    pDC
    Preparation
    Cell harvesting/tissue preparation method: Ficoll
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - saponin
    Gating Strategy
    FSC/SSC lymphocytes and then FSC-A/FSC-H singlets
    Username

    Abcam user community

    Verified customer

    投稿 Oct 04 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (tonsil)
    Specification
    tonsil
    Fixative
    Acetone
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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