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Synthetic peptide conjugated to BSA derived from within residues 450 to the C-terminus of Human IRF5.
(Peptide available as ab22411.)
Our Abpromise guarantee covers the use of ab21689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|CHIPseq||Use at an assay dependent concentration. PubMed: 25159141|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
GM-BMDMs from RelAFl/Fl mice were infected with either Cre (black bars) or Empty (white bars) adenovirus and stimulated with LPS (100ng/ml) for 2 hr or left unstimulated and used in ChIP analysis for IRF5 recruitment on Il-6 and Il-1a loci. Nuclear lysates were immunoprecipitated with 10µg of ab21689. Data indicate mean percent input relative to genomic DNA ± SD of a representative experiment.