Anti-IRF3 (phospho S386) 抗体 [EPR2346] (ab76493)
製品の概要
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製品名Anti-IRF3 (phospho S386) antibody [EPR2346]
IRF3 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2346] to IRF3 (phospho S386)
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由来種Rabbit
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特異性Detects IRF3 when phosphorylated on Serine 386.
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アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性交差種: Human
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免疫原
Phospho-specific peptide corresponding to residues surrounding Serine 386 of Human IRF3
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ポジティブ･コントロール
- MCF-7 cells treated with Calyculin A.
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特記事項
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2346
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab76493 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 47 kDa. |
ターゲット情報
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機能Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.
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組織特異性Expressed constitutively in a variety of tissues.
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配列類似性Belongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain. -
翻訳後修飾Constitutively phosphorylated on many serines residues. C-terminal serine/threonine cluster is phosphorylated in response of induction by IKBKE and TBK1. Ser-385 and Ser-386 may be specifically phosphorylated in response to induction. An alternate model propose that the five serine/threonine residues between 396 and 405 are phosphorylated in response to a viral infection. Phosphorylation, and subsequent activation of IRF3 is inhibited by vaccinia virus protein E3.
Ubiquitinated; ubiquitination involves RBCK1 leading to proteasomal degradation. Polyubiquitinated; ubiquitination involves TRIM21 leading to proteasomal degradation.
ISGylated by HERC5 resulting in sustained IRF3 activation and in the inhibition of IRF3 ubiquitination by disrupting PIN1 binding. The phosphorylation state of IRF3 does not alter ISGylation. -
細胞内局在Cytoplasm. Nucleus. Shuttles between cytoplasmic and nuclear compartments, with export being the prevailing effect. When activated, IRF3 interaction with CREBBP prevents its export to the cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 3661 Human
- Omim: 603734 Human
- SwissProt: Q14653 Human
- Unigene: 289052 Human
- Unigene: 75254 Human
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別名
- IIAE7 antibody
- Interferon regulatory factor 3 antibody
- IRF 3 antibody
see all
画像
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![Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)](http://a.abcam.com/ps/products/76/ab76493/Images/ab76493-249786-ab76493wb.jpg)
Western blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)All lanes : Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493) at 1/20000 dilution
Lane 1 : MCF7 cell lysate - untreated
Lane 2 : MCF7 cell lysate – treated with Calyculin A
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 47 kDaBlocking buffer - 5% NFDM/TBST
Diluting buffer - 1% BSA
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![Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)](http://a.abcam.com/ps/products/76/ab76493/Images/ab76493-249785-ab76493dotblot.jpg)
Dot Blot - Anti-IRF3 (phospho S386) antibody [EPR2346] (ab76493)Dot blot analysis of IRF3 single phospho peptide pS386 (lane 1) and IRF3 non-phospho peptide (lane 2) with ab76493 at 1/1000. Blocking and diluting buffer was 5% NFDM/TBST. The secondary antibody used was ab97051 Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/100,000.
プロトコール
参考文献
This product has been referenced in:
- Oh C et al. A central role for PI3K-AKT signaling pathway in linking SAMHD1-deficiency to the type I interferon signature. Sci Rep 8:84 (2018). Read more (PubMed: 29311560) »
- He X et al. ERRa negatively regulates type I interferon induction by inhibiting TBK1-IRF3 interaction. PLoS Pathog 13:e1006347 (2017). WB ; Human . Read more (PubMed: 28591144) »
レビューと Q&A
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