製品の概要

  • 製品名Anti-IRF1 antibody - ChIP Grade
    IRF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IRF1 - ChIP Grade
  • アプリケーション適用あり: IHC-P, ICC/IF, WB, Flow Cyt, ChIPmore details
  • 種交差性
    交差種: Dog, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 225 - 325 of Human IRF1.

    (Peptide available as ab27763.)

  • ポジティブ・コントロール
    • MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/225. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 21411754ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
ChIP Use at an assay dependent concentration. PubMed: 21757724

ターゲット情報

  • 機能Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and activates those genes. Acts as a tumor suppressor.
  • 関連疾患Defects in IRF1 are a cause of gastric cancer (GASC) [MIM:613659]; also called gastric cancer intestinal or stomach cancer. Gastric cancer is a malignant disease which starts in the stomach, can spread to the esophagus or the small intestine, and can extend through the stomach wall to nearby lymph nodes and organs. It also can metastasize to other parts of the body. The term gastric cancer or gastric carcinoma refers to adenocarcinoma of the stomach that accounts for most of all gastric malignant tumors. Two main histologic types are recognized, diffuse type and intestinal type carcinomas. Diffuse tumors are poorly differentiated infiltrating lesions, resulting in thickening of the stomach. In contrast, intestinal tumors are usually exophytic, often ulcerating, and associated with intestinal metaplasia of the stomach, most often observed in sporadic disease.
  • 配列類似性Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • 翻訳後修飾Sumoylation represses the transcriptional activity and displays enhanced resistance to protein degradation. Inactivates the tumor suppressor activity. Elevated levels in tumor cells. Major site is Lys-275. Sumoylation is enhanced by PIAS3 (By similarity). Desumoylated by SENP1 in tumor cells and appears to compete with ubiquitination on C-terminal sites.
    Ubiquitinated. Appears to compete with sumoylation on C-terminal sites.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Interferon regulatory factor 1 antibody
    • Interferon regulatory factor 1 isoform +I9 antibody
    • Interferon regulatory factor 1 isoform d78 antibody
    • Interferon regulatory factor 1 isoform delta4 antibody
    • Interferon regulatory factor 1 isoform delta7 antibody
    • IRF 1 antibody
    • IRF-1 antibody
    • IRF1 antibody
    • IRF1_HUMAN antibody
    • MAR antibody
    • MAR1 antibody
    see all

Anti-IRF1 antibody - ChIP Grade 画像

  • Anti-IRF1 antibody - ChIP Grade (ab26109) at 1 µg/ml + MOLT4 whole cell lysate (ab7912) at 20 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • Image courtesy of Human Protein Atlas. ab26109 staining IRF1 in human duodenum, showing nuclear staining of glandular cells. Paraffin embedded human duodenum tissue was incubated with ab26109 (1/225 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab26109 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ICC/IF image of ab26109 stained human HepG2 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab26109, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and MCF7 cells.

  • All lanes : Anti-IRF1 antibody - ChIP Grade (ab26109) at 1/250 dilution

    Lane 1 : Human AD293 whole cell lysate
    Lane 2 : Dog CPEK whole cell lysate

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 36 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-IRF1 antibody - ChIP Grade (ab26109) 使用論文

This product has been referenced in:
  • Schoggins JW  et al. A diverse range of gene products are effectors of the type I interferon antiviral response. Nature : (2011). ICC/IF ; Human . Read more (PubMed: 21478870) »
  • Harman AN  et al. HIV infection of dendritic cells subverts the IFN induction pathway via IRF-1 and inhibits type 1 IFN production. Blood 118:298-308 (2011). Flow Cyt ; Human . Read more (PubMed: 21411754) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse hepatocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Treatment 20 ug/ml poly(I:C)
Specification mouse hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Sep 18 2015

Application Western blot
Loading amount 30 cells
Gel Running Conditions Reduced Denaturing (4-20% polyacrylimde)
Sample Dog Cell lysate - whole cell (CPEK)
Specification CPEK
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 15 2014

Thank you very much for your interest our products

.
To our knowledge, ab26109 has not been tested in mouse . However by participating in our AbTrial program you can now use our products in an untested application or species without financ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Macrophages)
Specification Macrophages
Fixation Paraformaldehyde
Permeabilization Yes - Triton-x-100
Username

Dr. Ravikumar Muthuswamy

Verified customer

投稿 Mar 02 2012

Thank you for your call today and for your questions about ab26109. Please keep me updated about any new results or progress with this antibody, and let me know if you have any questions or if there is anything else we can do for you. Have a great week...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (primary epithelial cell)
Loading amount 10 µg
Specification primary epithelial cell
Treatment IFN gamma 2-4 hr
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Lori Manzel

Verified customer

投稿 Jul 23 2010

Thank you for your enquiry. An alignment of the peptide used to generate ab26109 with the chicken IRF1 sequence (primary accession number Q90876) shows only 23% identity. We do not predict this antibody to react with the chicken protein. If you h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"