製品の概要

  • 製品名Anti-IREB2 antibody
    IREB2 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to IREB2
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide:

    C-SIHYEGSEYKLSHGS

    , corresponding to internal sequence amino acids 489-503 of Human IREB2 (NP_004127.1).

  • ポジティブ・コントロール
    • Human Liver lysate Human kidney

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab106926 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 3 - 6 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).

ターゲット情報

  • 機能RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'-UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA.
  • 配列類似性Belongs to the aconitase/IPM isomerase family.
  • 翻訳後修飾Ubiquitinated and degraded by the proteasome in presence of high level of iron and oxygen. Ubiquitinated by a SCF complex containing FBXL5. Upon iron and oxygen depletion FBXL5 is degraded, preventing ubiquitination and allowing its RNA-binding activity.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ACO3 antibody
    • FLJ23381 antibody
    • IRE BP2 antibody
    • IRE-BP 2 antibody
    • IREB 2 antibody
    • IREB2 antibody
    • IREB2_HUMAN antibody
    • Iron regulatory protein 2 antibody
    • iron responsive element binding protein 2 antibody
    • Iron-responsive element-binding protein 2 antibody
    • IRP 2 antibody
    • IRP2 antibody
    • IRP2AD antibody
    • OTTHUMP00000185022 antibody
    see all

Anti-IREB2 antibody 画像

  • All lanes : Anti-IREB2 antibody (ab106926) at 0.3 µg/ml

    Lane 1 : Human Liver lysate in RIPA buffer
    Lane 2 : Human Liver lysate in RIPA buffer with immunizing peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 105 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab106926 at 3.8 µg/ml staining IREB2 in Human spleen by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

Anti-IREB2 antibody (ab106926) 使用論文

ab106926 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"