製品の概要

  • 製品名Anti-IRE1 (phospho S724) antibody
    IRE1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IRE1 (phospho S724)
  • 特異性ab48187 detects IRE1 alpha, phosphorylated (serine 724) protein.
  • アプリケーション適用あり: WB, IHC-P, ELISA, IP, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
    非交差種: Monkey
  • 免疫原

    Synthetic peptide corresponding to Human IRE1 (phospho S724).
    Database link: O75460
    (Peptide available as ab110445)

  • ポジティブ・コントロール
    • Glucose treated pancreatic islets and insulinoma cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 110 kDa.

Block with 3-5% BSA.

IHC-P 1/10 - 1/500.
ELISA 1/100 - 1/2000.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20477942

ターゲット情報

  • 機能Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • 組織特異性Ubiquitously expressed. High levels observed in pancreatic tissue.
  • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Anti-IRE1 (phospho S724) antibody 画像

  • All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution

    Lane 1 : HeLa cells 30 nM Calyculin A: AP-buffer
    Lane 2 : HeLa cells 30 nM Calyculin A: Alkaline Phosphatase

    Lysates/proteins at 20 µg per lane.

    Secondary
    infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 110 kDa

    The blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membranes were blocked for an hour. Membrane 2 was incubated with alkaline phosphatase (AP; 100 U per mL) for one hour, whilst membrane 1 was treated with AP-buffer only, before being  incubated with ab48187 (rabbit anti-IRE1 antibody diluted 1:2000) and loading control ab125247 (mouse anti-GAPDH antibody; diluted 1:10,000) for 24 hours at 4°C. Antibody binding was detected using infrared (IR)-labelled goat anti-rabbit (green) antibody and IR-labelled goat anti-mouse (red) at 1:10,000 dilutions for 1 hour at room temperature before imaging.

  • ab48187 staining IRE1 (phospho S724) in rat testis tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with formaldehyde, permeabilized using 0.1% Triton X-100 and then blocked with 2% BSA for 1 hour at 25°C, followed by incubation with the primary antibody at a 1/200 dilution, for 1 hour at 25°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

    See Abreview

  • Serially diluted ab48187 was bound to immobilised Phospho peptide (133861) - or Control peptide (1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 50000 times) and signal was developed with TMB substrate.

  • All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/1000 dilution

    Lane 1 : Min6 cells untreated
    Lane 2 : Min6 cells treated with glucose for 3 hours at 5 mM
    Lane 3 : Min6 cells treated with glucose for 3 hours at 20 mM

    Developed using the ECL technique

    Predicted band size : 110 kDa
  • All lanes : Anti-IRE1 (phospho S724) antibody (ab48187) at 1/2000 dilution

    Lane 1 : Cell lysate prepared from COS-7 Untransfected cells
    Lane 2 : Cell lysate prepared from COS-7 cells expressing wild type IRE1 alpha
    Lane 3 : Cell lysate prepared from COS-7 cells expressing kinase-dead IRE1 alpha


    Predicted band size : 110 kDa

Anti-IRE1 (phospho S724) antibody (ab48187) 使用論文

This product has been referenced in:
  • Kang K  et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep 6:22632 (2016). WB . Read more (PubMed: 26961159) »
  • Hyrskyluoto A  et al. Ubiquitin-specific protease-14 reduces cellular aggregates and protects against mutant huntingtin-induced cell degeneration: involvement of the proteasome and ER stress-activated kinase IRE1a Hum Mol Genet N/A:N/A (2014). Read more (PubMed: 24951540) »

See all 18 Publications for this product

Product Wall

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Application Western blot
Sample Human Cell lysate - whole cell (N87 cells)
Gel Running Conditions Reduced Denaturing (4-20% Tris Glycin gel)
Loading amount 800000 cells
Specification N87 cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

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投稿 Aug 03 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc1 pancreatic cancer cell line)
Permeabilization Yes - 0.25% TritonX-100 in PBS 10 minutes
Specification Panc1 pancreatic cancer cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
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Abcam user community

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投稿 Jan 19 2016

Application Western blot
Sample Mouse Tissue lysate - whole (retina)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 40 µg
Specification retina
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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投稿 Jul 20 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mesenteric artery)
Permeabilization No
Specification mesenteric artery
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Acetone
Username

Abcam user community

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投稿 Jul 06 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample Mouse Cell (retina)
Specification retina
Permeabilization Yes - Triton X-100
Fixative Paraformaldehyde
Username

Dr. Minzhong Yu

Verified customer

投稿 Jan 07 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (4-12% gel, TGS buffer)
Sample Mouse Tissue lysate - whole (Heart left ventricule)
Specification Heart left ventricule
Treatment Tunicamycin for 48h
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10)
Sample Human Cell lysate - whole cell (prostate cancer cell 22Rv1)
Specification prostate cancer cell 22Rv1
Treatment 300nM Thapsigargin for 6 and 12 hours
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (retina)
Loading amount 30 µg
Specification retina
Treatment 25mM high glucose
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Mr. Dongil Kim

Verified customer

投稿 Apr 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (LETO rat liver)
Loading amount 40 µg
Specification LETO rat liver
Gel Running Conditions Reduced Denaturing (7% SDS-PAGE gel)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Chrispina Hong

Verified customer

投稿 Jan 18 2013

1-10 of 22 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"