製品の概要

  • 製品名Anti-IRE1 antibody
    IRE1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to IRE1
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment: EEVINLVDQT SENAPTTVSR DVEEKPAHAP ARPEAPVDSM LKDMATIILS TFLLIGWVAF IITYPLSMHQ QQQLQHQQFQ KELEKIQLLQ QQQQQLPFHP , corresponding to amino acids 401-500 of Human IRE1 (AAC25991) with a 26 kDa tag

  • ポジティブ・コントロール
    • HeLa cell lysate, Human esophagus tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab87964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: Use at an assay dependent dilution.
    IHC-P: Use at a concentration of 1.5 µg/ml. Antigen retrieval is not essential but may optimise staining when the microwave method is used.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 110 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • 組織特異性Ubiquitously expressed. High levels observed in pancreatic tissue.
    • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated.
    • 細胞内局在Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 antibody 画像

    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + Immunogen (Recombinant protein) at 0.2 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-IRE1 antibody (ab87964) at 1 µg/ml + HeLa cell lysate at 50 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
    • ab87964 at 1.5µg/ml staining IRE1 in Human esophagus by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.

    Anti-IRE1 antibody (ab87964) 使用論文

    ab87964 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab87964.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"