The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1/8000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 110 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
Ubiquitously expressed. High levels observed in pancreatic tissue.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 KEN domain. Contains 1 protein kinase domain.
Xue LX et al. Neuroprotective effects of Activin A on endoplasmic reticulum stress-mediated apoptotic and autophagic PC12 cell death. Neural Regen Res12:779-786 (2017).
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