製品の概要

  • 製品名Anti-IRE1 antibody
    IRE1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to IRE1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Cow, Dog, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    C-PNAHGKIKAMISD

    , corresponding to internal sequence amino acids 699-711 of human IRE1 (NP_001424.3).

  • ポジティブ・コントロール
    • Human pancreas lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab77544 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報Peptide ELISA: Antibody detection limit dilution 1/8000.
    WB: Use at a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 110 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
    • 組織特異性Ubiquitously expressed. High levels observed in pancreatic tissue.
    • 配列類似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 1 KEN domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Autophosphorylated.
    • 細胞内局在Endoplasmic reticulum membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
      • Endoplasmic reticulum to nucleus signaling 1 antibody
      • Endoplasmic reticulum-to-nucleus signaling 1 antibody
      • Endoribonuclease antibody
      • ER to nucleus signaling 1 antibody
      • ERN 1 antibody
      • Ern1 antibody
      • ERN1_HUMAN antibody
      • hIRE 1p antibody
      • hIRE1p antibody
      • Inositol requiring 1 antibody
      • Inositol requiring 1, S. cerevisiae, homolog of antibody
      • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
      • Inositol requiring protein 1 antibody
      • inositol-requiring enzyme 1 antibody
      • Inositol-requiring protein 1 antibody
      • IRE 1 antibody
      • IRE 1a antibody
      • IRE 1P antibody
      • Ire1 alpha antibody
      • Ire1-alpha antibody
      • IRE1a antibody
      • Ire1alpha antibody
      • IRE1P antibody
      • MGC163277 antibody
      • MGC163279 antibody
      • Protein kinase/endoribonuclease antibody
      • RGD1559716 antibody
      • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
      see all

    Anti-IRE1 antibody 画像

    • Anti-IRE1 antibody (ab77544) at 0.03 µg/ml + human pancreas lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 110 kDa
      Observed band size : 110 kDa

    Anti-IRE1 antibody (ab77544) 使用論文

    ab77544 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77544.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"