製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IRE1
  • アプリケーション
    適用あり: WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide consisting of 16 amino acid from near the carboxy terminus (Human).

  • ポジティブ・コントロール
    • WB: A20 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab37073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 107 kDa (predicted molecular weight: 110 kDa).

Please note there is a smaller isoform of IRE1 in rat (predicted molecular weight:68.6kDa; accession number is EDM06401). Block for 2 hours at RT or overnight at 4C using 5% non-fat milk.

ICC/IF Use a concentration of 1 µg/ml.
ICC Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.
  • 組織特異性
    Ubiquitously expressed. High levels observed in pancreatic tissue.
  • 配列類似性
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 KEN domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
  • 細胞内局在
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Endoplasmic reticulum (ER) to nucleus signalling 1 antibody
    • Endoplasmic reticulum to nucleus signaling 1 antibody
    • Endoplasmic reticulum-to-nucleus signaling 1 antibody
    • Endoribonuclease antibody
    • ER to nucleus signaling 1 antibody
    • ERN 1 antibody
    • Ern1 antibody
    • ERN1_HUMAN antibody
    • hIRE 1p antibody
    • hIRE1p antibody
    • Inositol requiring 1 antibody
    • Inositol requiring 1, S. cerevisiae, homolog of antibody
    • Inositol requiring enzyme 1, S. cerevisiae, homolog of antibody
    • Inositol requiring protein 1 antibody
    • inositol-requiring enzyme 1 antibody
    • Inositol-requiring protein 1 antibody
    • IRE 1 antibody
    • IRE 1a antibody
    • IRE 1P antibody
    • Ire1 alpha antibody
    • Ire1-alpha antibody
    • IRE1a antibody
    • Ire1alpha antibody
    • IRE1P antibody
    • MGC163277 antibody
    • MGC163279 antibody
    • Protein kinase/endoribonuclease antibody
    • RGD1559716 antibody
    • Serine/threonine protein kinase/endoribonuclease IRE1 antibody
    see all

Anti-IRE1 antibody 画像



  • Predicted band size : 110 kDa
    Western Blot of rat brain lysate labeling IRE1 with Anti-IRE1 antibody (ab37073) at (1) 0.5 and (2) 1µg/ml.
  • Lane 1 : Anti-IRE1 antibody (ab37073) at 0.5 µg/ml
    Lane 2 : Anti-IRE1 antibody (ab37073) at 1 µg/ml
    Lane 3 : Anti-IRE1 antibody (ab37073) at 2 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate
    Lane 3 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Secondary
    goat anti-rabbit HRP at 1/10000 dilution

    Predicted band size : 110 kDa
    Observed band size : 107 kDa (why is the actual band size different from the predicted?)
  • ab37073, at 1 µg/ml, staining IRE1 in A20 cells with by ICC.

  • Immunocytochemistry/Immunofluorescence of IRE1p in A20 cells using ab37073 at 2 ug/ml.

Anti-IRE1 antibody (ab37073) 使用論文

This product has been referenced in:
  • Feng S  et al. NS5ABP37 inhibits liver cancer by impeding lipogenesis and cholesterogenesis. Cancer Sci 108:12-22 (2017). WB ; Human . Read more (PubMed: 27862769) »
  • Jiang Y  et al. Expression of inositol-requiring enzyme 1ß is downregulated in colorectal cancer. Oncol Lett 13:1109-1118 (2017). IHC-P ; Human . Read more (PubMed: 28454221) »

See all 25 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I will let you know as soon as I have any further information regarding the immunogen sequences fo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (CEC)
Loading amount
30 µg
Specification
CEC
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Apr 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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