製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IRAKM
  • 特異性
    Anti-IRAK-M has no cross response to IRAK or IRAK2.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Human
  • 免疫原

    Synthetic peptide:

    CSSKFSWDEYEQYKKE

    , corresponding to C terminal amino acids 581-596 of Human IRAKM (Peptide available as ab6231.)

  • ポジティブ・コントロール
    • Mouse spleen or rat kidney tissue lysate.
  • 特記事項


    Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • バッファー
    PBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    Purified IgG prepared by ion exchange chromatography. IRAK-M Antibody is affinity chromatography purified via peptide column.
  • 一次抗体 備考
    Interleukin-1 (IL-1) and lipopolysaccharide (LPS)induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor associated kinase (IRAK and IRAK2) mediates the activation of NF-kB by IL-1/Toll receptors (Wesche H et al.1999,Muzio M et al.1997), which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in theIRAK/Pelle family was recently identified and designated IRAK-M (Cao Z et al.1996). IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and thedominant negative mutants of IRAKs inhibit IL-1 orLPS induced NF-kB activation. Members inIRAK/Pelle family play a central role in IL-1R/TLRmediated inflammatory responses to cytokine IL-1and LPS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 20585389
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Can be blocked with IRAKM peptide (ab6231).

ターゲット情報

  • 機能
    Inhibits dissociation of IRAK1 and IRAK4 from the Toll-like receptor signaling complex by either inhibiting the phosphorylation of IRAK1 and IRAK4 or stabilizing the receptor complex.
  • 組織特異性
    Expressed predominantly in peripheral blood lymphocytes.
  • 関連疾患
    Defects in IRAK3 are associated with susceptibility to asthma-related traits type 5 (ASRT5) [MIM:611064]. Asthma-related traits include clinical symptoms of asthma, such as coughing, wheezing, dyspnea, bronchial hyperresponsiveness as assessed by methacholine challenge test, serum IgE levels, atopy, and atopic dermatitis.
  • 配列類似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASRT5 antibody
    • IL 1 receptor associated kinase M antibody
    • IL-1 receptor-associated kinase M antibody
    • IL1 receptor associated kinase M antibody
    • Interleukin 1 receptor associated kinase 3 antibody
    • Interleukin 1 receptor associated kinase M antibody
    • Interleukin-1 receptor-associated kinase 3 antibody
    • IRAK M antibody
    • IRAK-3 antibody
    • IRAK-M antibody
    • Irak3 antibody
    • IRAK3_HUMAN antibody
    see all

画像

  • All lanes : Anti-IRAKM antibody (ab8116) at 1 µg/ml

    Lane 1 : Mouse spleen tissue lysates
    Lane 2 : Rat liver tissue lysate


    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Western blot analysis of IRAKM in mouse spleen (M) and rat liver (R) tissue lysates with anti-IRAKM at 1 µg /ml.
  • Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 µg/ml.

  • ab8116 at 2µg/ml staining IRAKM in rat liver cells by IHC  

  • ab8116 staining IRAKM in murine lung macrophages by Immunocytochemistry/ Immunofluorescence.<.br>
    The cells were permeablized with a 1:1 mixture of methanol and acetone. After washing with PBS, ab8116 was applied and the cells incubated for one hour. After washing, antibodies against the primary antibody labeled with FITC were applied to the cells and incubated for one hour. The cell were then mounted in DAPI mounting media and viewed on the fluorescent microscope.
  • Immunofluorescence of IRAK-M in Rat Liver cells using ab8116 at 10 ug/ml.

参考文献

This product has been referenced in:
  • Al-Qahtani AA  et al. Middle east respiratory syndrome corona virus spike glycoprotein suppresses macrophage responses via DPP4-mediated induction of IRAK-M and PPAR?. Oncotarget 8:9053-9066 (2017). Read more (PubMed: 28118607) »
  • Udgata A  et al. Transduction of Functionally Contrasting Signals by Two Mycobacterial PPE Proteins Downstream of TLR2 Receptors. J Immunol 197:1776-87 (2016). Read more (PubMed: 27481848) »

See all 10 Publications for this product

レビューと Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 10 2014

Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Dec 23 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Tissue lysate - whole (monocyte-derived macrophage)
Loading amount
30 µg
Specification
monocyte-derived macrophage
Treatment
100 µg/ml muramyl dipeptide for 48 hrs
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Jan 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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