製品の概要
- 製品名Anti-IRAK4 antibody [Y279]
IRAK4 一次抗体 製品一覧 - 製品の詳細Rabbit monoclonal [Y279] to IRAK4
- アプリケーション適用あり:
ICC, IHC-P, WB, Flow Cyt, ICC/IFmore details
適用なし: IP - 種交差性交差種: Human
- 免疫原
A synthetic peptide corresponding to residues in the N-term of human IRAK-4.
- ポジティブ･コントロール
- Jurkat cell lysate. IHC-P: FFPE human spleen tissue sections.
- 特記事項
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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Concentration information loading... - 精製度IgG fraction
- ポリ/モノモノクローナル
- クローン名Y279
- アイソタイプIgG
- 研究分野
関連製品
- Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab32511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/50 - 1/100. | |
| IHC-P | Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 52 kDa. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
ターゲット情報
- 機能Required for the efficient recruitment of IRAK1 to the IL-1 receptor complex following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Phosphorylates IRAK1.
- 関連疾患Defects in IRAK4 are the cause of recurrent isolated invasive pneumococcal disease type 1 (IPD1) [MIM:610799]. Recurrent invasive pneumococcal disease (IPD) is defined as two episodes of IPD occurring at least 1 month apart, whether caused by the same or different serotypes or strains. Recurrent IPD occurs in at least 2% of patients in most series, making IPD the most important known risk factor for subsequent IPD.
Defects in IRAK4 are the cause of IRAK4 deficiency (IRAK4D) [MIM:607676]. IRAK4 deficiency causes extracellular pyogenic bacterial and fungal infections in otherwise healthy children. - 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 death domain.
Contains 1 protein kinase domain. - Information by UniProt
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参照データベース
- Entrez Gene: 51135 Human
- Omim: 606883 Human
- SwissProt: Q9NWZ3 Human
- Unigene: 138499 Human
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別名
- IL-1 receptor-associated kinase 4 antibody
- Interleukin 1 receptor associated kinase 4 mutant form 1 antibody
- Interleukin-1 receptor-associated kinase 4 antibody
see all
Anti-IRAK4 antibody [Y279] 画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [Y279] (ab32511)](http://a.abcam.com/ps/products/32/ab32511/Images/ab32511-207009-ab32511Spleen1edit.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IRAK4 antibody [Y279] (ab32511)IHC image of ab32511 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32511, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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![Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [Y279] (ab32511)](http://a.abcam.com/ps/products/32/ab32511/Images/ab32511-268514-ab32511ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IRAK4 antibody [Y279] (ab32511)Immunocytochemistry/Immunofluorescence analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling IRAK4 with ab32511 at 1/100 dilution. Cells were fixed with 100% methanol. ab150077, an AlexaFluor® 488 conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, anti-alpha tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. Nuclei counterstained with DAPI (blue).
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![Western blot - IRAK4 antibody [Y279] (ab32511)](http://a.abcam.com/ps/products/32/ab32511/Images/ab32511wb.gif)
Western blot - IRAK4 antibody [Y279] (ab32511)Anti-IRAK4 antibody [Y279] (ab32511) at 1/500 dilution + Jurkat cell lysate
Observed band size : 52 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-IRAK4 antibody [Y279] (ab32511)](http://a.abcam.com/ps/products/32/ab32511/Images/ab32511-269688-ab32511FC1.jpg)
Flow Cytometry - Anti-IRAK4 antibody [Y279] (ab32511)Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling IRAK4 with purified ab32511 at 1/110 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Anti-IRAK4 antibody [Y279] (ab32511) 使用論文
ab32511 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"