製品の概要

  • 製品名
  • 製品の詳細
    Mouse monoclonal to IRAK2
  • アプリケーション
    適用あり: ELISA, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Orangutan
  • 免疫原

    Recombinant fragment with tag: KPEKPLAASV RKAEDEQEEG QPVRMATFPG PGSSPARAHQ PAFLQPPEED APHSLRSDLP TSSDSKDFST SIPKQEKLLS LAGDSLFWSE ADVVQATDDF , corresponding to amino acids 111-210 of Human IRAK2

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab66017 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
IP
  • 追加情報
    ELISA: Use at an assay dependent dilution. Sandwich ELISA: The detection limit for recombinant tagged IRAK2 is approximately 0.03ng/ml as a capture antibody.
    WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
    • 組織特異性
      Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
    • 配列類似性
      Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
      Contains 1 death domain.
      Contains 1 protein kinase domain.
    • ドメイン
      The protein kinase domain is predicted to be catalytically inactive.
    • Information by UniProt
    • 参照データベース
    • 別名
      • il1rak2 antibody
      • Interleukin 1 receptor associated kinase 2 antibody
      • Interleukin 1 receptor associated kinase like 2 antibody
      • Interleukin-1 receptor-associated kinase-like 2 antibody
      • IRAK 2 antibody
      • IRAK-2 antibody
      • Irak2 antibody
      • IRAK2_HUMAN antibody
      • MGC150550 antibody
      see all

    画像

    • Anti-IRAK2 antibody (ab66017) at 1 µg/ml + HeLa cell lysate at 50 µg/ml

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 65 kDa
      Observed band size : 65 kDa

    参考文献

    ab66017 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunoprecipitation
    Immuno-precipitation step
    Protein G
    Sample
    Human Cell lysate - whole cell (HEK293)
    Specification
    HEK293
    Total protein in input
    1000 µg
    Username

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    Verified customer

    投稿 Nov 07 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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