製品の概要

  • 製品名Anti-IRAK2 antibody
    IRAK2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IRAK2
  • 特異性Anti-IRAK2 has no cross response to IRAK.
  • アプリケーション適用あり: ICC/IF, WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 546-564 of Human IRAK2.

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa or K562 cells.
  • 特記事項


    The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度IgG fraction
  • 特記事項(精製)Purified IgG was prepared by affinity chromatography.
  • 一次抗体 備考The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-kB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2 (1). Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-kB activation. IRAKs also associate with Toll-like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-kB activation (2,3).Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab6148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 65 kDa.Can be blocked with IRAK2 peptide (ab6232).
IP Use at 1 µg/mg of lysate.

ターゲット情報

  • 機能Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • 組織特異性Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • 配列類似性Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • ドメインThe protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • 参照データベース
  • 別名
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

Anti-IRAK2 antibody 画像

  • All lanes : Anti-IRAK2 antibody (ab6148) at 2 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate


    Predicted band size : 65 kDa
    Observed band size : 65 kDa
  • ab6148 at 2µg/ml staining IRAK2 in Hela cells by ICC/IF

  • Immunofluorescence of IRAK-2 in Hela cells using ab6148 at 10 ug/ml.

Anti-IRAK2 antibody (ab6148) 使用論文

ab6148 has not yet been referenced specifically in any publications.

Product Wall

Application Immunoprecipitation
Immuno-precipitation step Protein G
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Total protein in input 1000 µg
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投稿 Nov 07 2013

We do not have any reports of this antibody being used for immunoprecipitation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"