製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IRAK2
  • 特異性
    ab25957 recognises IRAK2 but does not cross-react with IRAK.
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Synthetic peptide, corresponding to 16 C terminal amino acids of Mouse IRAK2a isoform. This sequence is common to all four isoforms.

  • ポジティブ・コントロール
    • Whole cell lysate from A-20 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab25957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 65 kDa.

ターゲット情報

  • 機能
    Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization.
  • 組織特異性
    Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes.
  • 配列類似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
    Contains 1 death domain.
    Contains 1 protein kinase domain.
  • ドメイン
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • 参照データベース
  • 別名
    • il1rak2 antibody
    • Interleukin 1 receptor associated kinase 2 antibody
    • Interleukin 1 receptor associated kinase like 2 antibody
    • Interleukin-1 receptor-associated kinase-like 2 antibody
    • IRAK 2 antibody
    • IRAK-2 antibody
    • Irak2 antibody
    • IRAK2_HUMAN antibody
    • MGC150550 antibody
    see all

画像

  • Lane 1 : Anti-IRAK2 antibody (ab25957) at 0.5 µg/ml
    Lane 2 : Anti-IRAK2 antibody (ab25957) at 1 µg/ml
    Lane 3 : Anti-IRAK2 antibody (ab25957) at 2 µg/ml

    Lane 1 : A-20 whole cell lysate
    Lane 2 : A-20 whole cell lysate
    Lane 3 : A-20 whole cell lysate


    Predicted band size : 65 kDa

プロトコール

参考文献

ab25957 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Nov 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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