IRAK overexpression 293T lysate (whole cell) (ab94046)

製品の概要

  • 製品名
    IRAK overexpression 293T lysate (whole cell)
  • 特記事項
    ab94046 is a 293T cell transfected lysate in which Human IRAK has been transiently over-expressed using a pCMV-IRAK plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details on how the transfected lysate was prepared view preparation notes
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. PTM: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. Contains 1 protein kinase domain. Tissue specificity: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

アプリケーション

Our Abpromise guarantee covers the use of ab94046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94046 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-IRAK antibody (ab55316) at 1/500 dilution

    Lane 1 : IRAK overexpression 293T lysate (whole cell) (ab94046)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94046 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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