The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 195 kDa (predicted molecular weight: 189 kDa).
1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/250.
追加情報Is unsuitable for Flow Cyt or IP.
機能Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
組織特異性Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
ドメインRegions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
翻訳後修飾Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
IQ motif containing GTPase activating protein 1 antibody
Ras GTPase activating like protein 1 antibody
Ras GTPase-activating-like protein IQGAP1 antibody
RasGAP-like with IQ motifs antibody
Anti-IQGAP1 antibody [EPR5221] 画像
Western blot - Anti-IQGAP1 antibody [EPR5221] (ab109292)
Predicted band size : 189 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: IQGAP1 knockout HAP1 cell lysate (20 µg) Lane 3: HEK293 cell lysate (20 µg) Lane 4: HeLa cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab109292 observed at 190 kDa. Red - loading control, ab7291, observed at 52 kDa. ab109292 was shown to specifically react with IQGAP1 when IQGAP1 knockout samples were used. Wild-type and IQGAP1 knockout samples were subjected to SDS-PAGE. ab109292 and ab7291 (loading control to alpha tubulin) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Western blot - IQGAP1 antibody [EPR5221] (ab109292)
All lanes : Anti-IQGAP1 antibody [EPR5221] (ab109292) at 1/1000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Cos-1 cell lysate Lane 3 : HUVEC cell lysate Lane 4 : Jurkat cell lysate