製品の概要

  • 製品名Anti-IQGAP1 antibody [0.T.72]
    IQGAP1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [0.T.72] to IQGAP1
  • 特異性Recognizes human IQGAP1 at 185kD. (Does not recognize IQGAP2).
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment by a Glutaraldehyde linker, corresponding to C terminal amino acids 863-1657 of Human IQGAP1.

  • ポジティブ・コントロール
    • HepG2 RIPA cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
  • 追加情報IP: Use at a concentration of 4 µg/ml (immunoprecipitated IQGAP1 from 500µg of a HepG2 RIPA lysate).
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 184 kDa. A goat anti-mouse secondary antibody conjugated to HRP can be used as secondary antibody together with a chemiluminescence detection system.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
    • 組織特異性Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
    • 配列類似性Contains 1 CH (calponin-homology) domain.
      Contains 4 IQ domains.
      Contains 1 Ras-GAP domain.
      Contains 1 WW domain.
    • ドメインRegions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
    • 翻訳後修飾Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser-1443 phosphorylation enhances the ability to promote neurite outgrowth.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • HUMORFA01 antibody
      • IQ motif containing GTPase activating protein 1 antibody
      • IQGA1_HUMAN antibody
      • Iqgap1 antibody
      • KIAA0051 antibody
      • p195 antibody
      • Ras GTPase activating like protein 1 antibody
      • Ras GTPase-activating-like protein IQGAP1 antibody
      • RasGAP-like with IQ motifs antibody
      • SAR1 antibody
      see all

    Anti-IQGAP1 antibody [0.T.72] (ab33542) 使用論文

    This product has been referenced in:
    • Liu C  et al. IQGAP1 suppresses TßRII-mediated myofibroblastic activation and metastatic growth in liver. J Clin Invest 123:1138-56 (2013). Human . Read more (PubMed: 23454766) »
    • Liang S  et al. Honokiol inhibits HepG2 migration via down-regulation of IQGAP1 expression discovered by a quantitative pharmaceutical proteomic analysis. Proteomics 10:1474-83 (2010). WB ; Human . Read more (PubMed: 20127691) »

    See all 3 Publications for this product

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    There are currently no Abreviews or Questions for ab33542.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"