製品の概要

  • 製品名Anti-IPP antibody
    IPP 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to IPP
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 432-481 (CCEMQGLIYV IGGISNEGIE LRSFEVYDPL SKRWSPLPPM GTRRAYLGVA) of Human IPP (NP_005888).

  • ポジティブ・コントロール
    • Hela cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/1562500.

ターゲット情報

  • 関連性Intracisternal A particle promoted polypeptide (IPP) is a member of the kelch family of proteins, characterized by a 50 amino acid repeat which interacts with actin. The kelch domain of IPP consists of six C terminal tandem arranged repeats. IPP may play a role in organizing the actin cytoskeleton. IPP also contains an N terminal POZ protein protein interaction domain. The POZ domain (also called BTB domain) is present near the N terminus of a fraction of zinc finger proteins and in proteins that contain the pfam01344 motif such as kelch and pox virus proteins. The BTB/POZ domain mediates homomeric dimerization and in some instances heteromeric dimerization. POZ domains from several zinc finger proteins have been shown to mediate transcriptional repression and to interact with components of histone deacetylase co repressor complexes including N-coR and SMRT.
  • 細胞内局在Cytoplasm, cytoskeleton.
  • 参照データベース
  • 別名
    • Actin binding protein IPP antibody
    • Intracisternal A particle promoted polypeptide antibody
    • IPP POZ antibody
    • Kelch like protein 27 antibody
    • KLHL27 antibody
    • MIPP protein antibody
    see all

Anti-IPP antibody 画像

  • Anti-IPP antibody (ab81692) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

Anti-IPP antibody (ab81692) 使用論文

ab81692 has not yet been referenced specifically in any publications.

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