製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to IPMK
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant protein fragment, corresponding to a region within amino acids 26 and 265 of Human IPMK (NP_689416).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLaS3, HepG2 and Raji cell lysates for WB and HeLa cells for IF.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

画像

  • Anti-IPMK antibody (ab96753) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 47 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96753 at 1/200 dilution. Lower image merged with DNA probe.

参考文献

ab96753 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (FAO rat hepatoma cells)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
25 µg
Treatment
100 nM insulin for 5 minutes
Specification
FAO rat hepatoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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投稿 Sep 29 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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